中国现代中药2016，Vol.18Issue(7)：862-865,4.DOI:10.13313/j.issn.1673-4890.2016.7.013
女贞子中齐墩果酸含量测定及促进葡萄糖消耗体外细胞实验研究
Determination of Content Oleanolic Acid in Ligustri Lucidi Fructus by HPLC and Its Effect on Promoting the Glucose Consumption in vitro
摘要
Abstract
Objective:To measure the content of oleanolic acid in 10 different batches of Ligustri lucidi Fructus and study the hypoglycemic effect of the ethanol extract of Ligustri lucidi Fructus in vitro.Methods:Ligustri lucidi Fructus were extracted by 10 folds of 70% ethanol,1 h a time,and extracted several times until oleanolic acid was undetectable,the extracts were then merged and enriched.The content of oleanolic acid was determined by HPLC.The hypoglycemic effect of the ethanol extract of Ligustri lucidi Fructus was studied by human liver cancer cell HepG2 cells.Results:The content of oleanolic acid of the different batches of Ligustri lucidi Fructus varied and was above 0.50%.Different concentrations of extract showed the effect of promoting cells to use glucose,and the effect increased with the concentration within a certain scope. Conclusion:The ethanol extract of Ligustri lucidi Fructus has the effect of promoting the glucose consumption,it deserves for further study .关键词
女贞子/齐墩果酸/葡萄糖消耗Key words
Ligustri lucidi Fructus/Oleanolic acid/glucose consumption引用本文复制引用
孙京海,潘晴,李凌军,马鲁豫..女贞子中齐墩果酸含量测定及促进葡萄糖消耗体外细胞实验研究[J].中国现代中药,2016,18(7):862-865,4.基金项目
“十二五”重大新药创制国家科技重大专项 （）
