北京林业大学学报2016，Vol.38Issue(7)：25-32,8.DOI:10.13332/j.1000-1522.20150499
C14族R2 R3-MYB基因调控杨树抗锈菌过敏性反应
Impact of clade 14 R2 R3-MYB genes on hypersensitive response of the poplar infected with Melampsora larici-populina
摘要
Abstract
Leaf rust caused by Melampsora larici-populina is the main disease affecting poplar plantations, causing severe economic losses. To explore the regulatory effects of clade 14 R2R3-MYB genes on hypersensitive response of the poplar infected with M. larici-populina, leaves of two poplar species were inoculated by M. larici-populina E4 pathotypes to observe the symptom and score the infectious index. In addition, changes of the gene expression level of six clade 14 R2R3-MYB genes were analyzed. After seven days of inoculation, the number of uredinium on Populus deltoides í P. trichocarpa ( less susceptible to M. larici-populina E4 pathotypes ) leaves was significantly less than that on P. íeuramericana ( very susceptible to M. larici-populina E4 pathotypes) . On the fourth day of inoculation, symptoms of hypersensitive reaction ( HR) were observed on leaves of P. deltoidesíP. trichocarpa, but not on Populusíeuramericana leaves. It turns out that HR can prevent the formation of uredinium and reduce the susceptibility of poplar. The change trend of R2R3-MYB gene expression level of the two poplar species was contrary. It is estimated that poplar clade 14 R2R3-MYB genes may be positive regulators of the HR in host in response to rust attack. This study provides a theoretical foundation for further studying these transcriptional factors in the control of the HR and the possible usage of these genes for the production of rust-resistant poplar.关键词
转录因子/转录调控/美洲黑杨伊毛果杨/欧美杨/落叶松-杨栅锈菌Key words
transcription factor/transcriptional regulation/Populus deltoides í P. trichocarpa/P. íeuramericana/Melampsora larici-populina分类
农业科技引用本文复制引用
牛春阳,王峰,李丹蕾,陈俏丽,张瑞芝..C14族R2 R3-MYB基因调控杨树抗锈菌过敏性反应[J].北京林业大学学报,2016,38(7):25-32,8.基金项目
国家自然科学基金项目(31500524)、中央高校基本科研业务费专项(2572015EY02、2572015CA09、2572016CA04)、黑龙江省博士后基金(LBH-Z11248)、高等学校博士学科点专项科研基金(博士点新教师基金20100062120002)。 （31500524）
