北京林业大学学报2016，Vol.38Issue(7)：33-39,7.DOI:10.13332/j.1000-1522.20160071
胞外ATP对胡杨细胞囊泡运输的影响
Effect of eATP on vesicular trafficking in Populus euphratica cells
摘要
Abstract
Being as a significant signal molecule, extracellular ATP ( eATP) participates in the processes of plant growth, development and stress responses. These processes are shown to be associated with vesicular trafficking, however, the function of eATP on vesicular trafficking is not yet clear. Here, we explored the effect of eATP on vesicular trafficking in Populus euphratica cells using FM1-43 , a selective fluorescent dye for membrane binding. FM1-43 staining revealed that 50 μmol/L eATP had no obvious effect on endocytosis in P. euphratica cells. However, the endocytosis was inhibited by higher dosages of eATP (200, 400μmol/L), and the inhibition effect was associated with increasing eATP concentrations. Moreover, high dosages of eATP (200, 400μmol/L) also inhibited exocytosis in P. euphratica cells. In contrast to eATP, ADP ( 50, 200, 400 μmol/L ) did not exert a significant impact on vesicular trafficking. This suggests that the inhibitory effect of high concentrations of eATP was not caused by the hydrolyzing product of eATP. Pharmacological experiments showed that the ATP receptor inhibitor, PPADS ( 100 μmol/L ) , could block the inhibitory effect of high eATP on vesicular trafficking. We conclude that eATP suppresses vesicular trafficking by a purine receptor-mediated signal pathway. However, another purine receptor inhibitor, suramin (100μmol/L) , has no obvious inhibitory effect on eATP signaling. Thus it could be inferred that other kinds of P2X cognate receptors mediate inhibitory effect of high eATP on vesicular trafficking. Collectively, eATP can regulate vesicular trafficking in P. euphratica cells, but high concentrations of eATP display a negative regulation on vesicular trafficking.关键词
胞外ATP/FM1-43/胡杨细胞/囊泡运输/胞吞/胞吐Key words
extracellular ATP/FM1-43/Populus euphratica cells/vesicular trafficking/endocytosis/exocytosis分类
农业科技引用本文复制引用
孙苑玲,邓澍荣,邓佳音,张会龙,尧俊,赵瑞,陈少良..胞外ATP对胡杨细胞囊泡运输的影响[J].北京林业大学学报,2016,38(7):33-39,7.基金项目
国家自然科学基金项目(31270654、31570587)、教育部科学技术研究(科学技术类)项目(113013A)、人事部留学人员科技活动项目(2012001)、高等学校学科创新引智计划项目(111 Project, B13007)、教育部创新团队发展计划项目(IRT13047)。 （31270654、31570587）
