动物医学进展2016，Vol.37Issue(7)：7-12,6.
2012年－2014年闽西地区 PRRSV 流行株ORF5基因遗传变异分析
Genetic Variation of ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus Isolates from Western Fujian in 2012-2014
摘要
Abstract
The result showed that 10 PRRSV isolates belonged to the North American genotype,the lengths of the nine ORF5 sequences were 603 nucleotides (FJ05 is 600 nucleotides).Ten isolates displayed a nucleotide identity ranging from 88.4% to 99.3% among themselves,they shared 88.3%-98.8% homolo-gy with VR2332,MLV and CH-1a;88.9%-99.0% with JXA1,WUH1 and HUN4,and they shared only 63.8-65.0% with LV.High variable occurred at positions of 9-40 aa and 58-62 aa of GP5.Amino acid se-quence alignments revealed that 10 isolates shared 88.5%-99.5% among themselves,they shared 87.5%-98.5% homology with VR-2332,MLV and CH-1a,88.0%-99.5% with JXA1,WUH1 and HUN4,howev-er,they shared only 56.6-59.2% with LV.The phylogenetic tree analysis showed that 10 isolates are divid-ed into three subgroups,8 strains (8/10,80%)belonged to subgroup Ⅰ,which are closely related to the JXA1 strain.The FJ01 strain (1/10,10%)belonged to subgroup Ⅱ,which is closely related to the VR-2332,FJ03 strain (1/10,10%)belong to subgroup Ⅲ,which is closely related to the HB-1sh/2002 (the intersubgenotype).These results showed that PRRSV have been shown to undergo remarkable genetic di-versity.关键词
猪繁殖与呼吸综合征病毒/ORF5 基因/反转录-聚合酶链反应/遗传变异Key words
Porcine reproductive and respiratory syndrome virus/ORF5 gene/RT-PCR/genetic variation分类
农业科技引用本文复制引用
黄春芳,刘建奎,龚丽珍,杨炳辉,曾建平,陈安妮,陈小燕..2012年－2014年闽西地区 PRRSV 流行株ORF5基因遗传变异分析[J].动物医学进展,2016,37(7):7-12,6.基金项目
福建省大学生创新创业计划训练项目(201511312057) （201511312057）
省属高校科研课题(JK2015049) （JK2015049）
福建省自然科学基金项目(2016J01 168) （2016J01 168）
福建省科技重大专项(2014NZ0002-3) （2014NZ0002-3）
