动物医学进展2016，Vol.37Issue(7)：34-37,38,5.
鸡传染性支气管炎病毒血凝抑制试验抗原的研制
Development of HI Test Antigen of Avian Infectious Bronchitis Virus
摘要
Abstract
In order to determine the serum antibody level of avian infectious bronchitis virus (IBV)in the inactivated vaccine,the preparation method of HI test antigen of IBV was established.The widest spectrum of IBV M41 strain was selected and proliferated in allantoic fluid of SPF chick embryos for 36 h,and then centrifuged in 4℃ and 12 000 r/min for 10 minutes,and last concentrated 100 fold by the polyethylene gly-col (PEG)20000 dialysis bag.Rabbit Clostridium perfringens type A standard Chinese strain (C57-1 strain)was first cultured for 18 h,then centrifuged supernatant in 4℃ and 12 000 r/min for 10 minutes, and then concentrated 5 fold by the PEG20000 dialysis bag,and last filtered by 0.20 μm membrane.The IBV HI antigen was made by mixing the IBV virus fluid and type A Clostridium perfringens liquid (con-taining alpha toxin)according to a certain proportion,then by the constant temperature of 37℃ for 2 h,and then in 4℃ for 48 h.It was showed that the prepared IBV HI test antigen had high titer and good stability, and could successfully replace imported antigen by using for the HI detection of serum antibody and egg yolk antibody in the sera of chickens immunized with IBV vaccine by a large number of experimental re-sults.关键词
传染性支气管炎病毒/血凝抑制试验/A型产气荚膜梭菌Key words
Infectious bronchitis virus/HI test/Clostridium perfringens type A分类
农业科技引用本文复制引用
庄金秋,梅建国,祖立闯,苗立中,沈志强..鸡传染性支气管炎病毒血凝抑制试验抗原的研制[J].动物医学进展,2016,37(7):34-37,38,5.基金项目
山东省重点研发计划项目(2015GSF121027) （2015GSF121027）
滨州市科技发展计划项目(2015ZC0109) （2015ZC0109）
