动物营养学报2016，Vol.28Issue(8)：2448-2457,10.DOI:10.3969/j.issn.1006-267x.2016.08.016
大豆异黄酮对肥胖大鼠肠道瘦素介导 Janus激酶／信号转导及转录激活因子信号转导通路的影响
Effects of Soybean Isoflavone on Signaling Transduction Path of Leptin-Dependent Janus Kinase/Signal Transduction and Activator of Transcription in Intestine of Obese Rats
摘要
Abstract
This study was conducted to study the regulatory effect of signaling transduction path of leptin⁃de⁃pendent Janus kinase ( JAK )/signal transduction and activator of transcription ( STAT ) in intestine of obese rats treated with soybean isoflavone ( SIF) , and to discuss the intervention mechanism of SIF on obese rats. Eighty SD male rats with the age of 5 weeks were randomly divided into basal diet group ( 12 rats) and high fat diet group ( 68 rats) after adapted to the environment for 1 week, and the rats in basal diet group and high fat diet group were fed with basal diet and high fat diet, respectively. After feeding 8 weeks, the food⁃induced obese rat models were successfully established in high fat diet group. Forty obese rats were selected to divide in⁃to 4 groups which were SIF interfering low, medium and high dose groups and obesity control group, and each group had 10 rats. The rats in low, medium and high dose groups were given 50, 150 and 450 mg/kg BW SIF, respectively, and the rats in basal diet group and obesity control group were given solvent agent through stomach for 5 weeks. During the experiment, the body weight of rats was measured, and the expression and distribution of obese receptor b ( OB⁃Rb) , Janus kinase 2 ( JAK2) , phosphorylated signal transducer and acti⁃vator of transcription 3 ( p⁃STAT3) , suppressor of cytokine signaling 3 ( SOCS3) and neuropeptide Y ( NPY) were detected by immunohistochemical staining of strept avidin⁃biotin complex ( SABC) method. The results showed as follows: the body weight of basal diet group was extremely significantly lower than that of obesity control group at each period ( P<0.01) . After 5 weeks of SIF intervention, the body weight of SIF intervention groups was decreased compared with obesity control group and showed a dose⁃dependence relationship, and the middle and high dose groups were extremely significantly lower than obesity control group ( P<0.01) . OB⁃Rb, JAK2, p⁃STAT3, SOCS3 and NPY of rats of each group all existed in duodenum, jejunum, ileum, colon and rectum. Compared with basal diet group, the expression levels of OB⁃Rb, JAK2, p⁃STAT3, SOCS3 and NPY at different intestine segments of rats of obesity control group were significantly decreased (P<0.05). Com⁃pared with obesity control group, the expression levels of OB⁃Rb, JAK2, p⁃STAT3 and NPY at different in⁃testine segments of rats of SIF intervention groups were obviously increased, and overall showed a dose⁃de⁃pendence relationship. The results suggest that SIF can increase the expression of intestinal OB⁃Rb, activate JAK, accelerate STAT phosphorylation, improve energy metabolism, attenuate leptin resistance, and then play a role on weight loss of obese rats.关键词
大豆异黄酮/免疫组织化学SABC染色法/OB-Rb/JAK2/p-STAT3/SOCS3/NPY/肥胖大鼠Key words
soy isoflavone/immunohistochemical staining of SABC method/OB-Rb/JAK2/p-STAT3/SOCS3/NPY/obese rat分类
农业科技引用本文复制引用
李立科,罗启慧,唐秀莹,黄超,陈晓林,刘文涛,陈正礼..大豆异黄酮对肥胖大鼠肠道瘦素介导 Janus激酶／信号转导及转录激活因子信号转导通路的影响[J].动物营养学报,2016,28(8):2448-2457,10.基金项目
国家科技支撑计划课题（2014BAI03B01）；国家重大科学仪器设备开发专项（2013YQ49085906）；四川省青年科技创新研究团队项目 （）
