动物营养学报2016，Vol.28Issue(8)：2485-2496,12.DOI:10.3969/j.issn.1006-267x.2016.08.020
茶皂素对奶牛瘤胃发酵及瘤胃微生物区系的影响
Effects of Tea Saponin on Rumen Fermentation and Rumen Microflora of Dairy Cows
摘要
Abstract
This experiment was conducted to study the effects of tea saponin on rumenfermentation and rumen microflora of dairy cows. Twelve healthy Holstein cows with similar weight were used, and randomly divided into four groups, all were fed a basal diet and drenched with 0 ( control) , 20, 30 and 40 g/d of tea saponin in different groups. Tea saponin and water were mixed for drenching. Pre experimental period went 14 days, and the trial period went 35 days. Rumen fluid was collected with oral sampler at 1 h before morning feeding every 7 days during the trail period for determination of rumen fermentation parameters and rumen microorganisms (real⁃time quantitative PCR method). The results showed as follows: 1) compared with control group, tea saponin significantly reduced rumen fluid pH (30 and 40 g/d groups), ammonia nitrogen concentration (20, 30 and 40 g/d groups) (P<0.05), which were not exceeded the normal range values, but significantly in⁃creased microbial protein (30 and 40 g/d groups), propionic acid (20, 30 and 40 g/d groups) and butyric acid concentrations (20, 30 and 40 g/d groups) (P<0.05), and microbial protein concentration of 30 g/d tea saponin group increased 20. 20%; however, tea saponin did not significantly affected total volatile fatty acid and acetic acid concentrations ( P>0.05) . 2) Compared with control group, the contents of rumen fluid proto⁃zoa and Butyrivibrio fibrisolvens of tea saponin groups were significantly decreased ( P<0.05) , but no signifi⁃cant changes on methanogens, Ruminococcus albus, Fibrobacter succinogenes, Ruminococcus flavefaciens and fungi contents were found ( P>0.05) . In conclusion, the supplementation of tea saponin can improve the pat⁃tern of rumen fermentation and has a significant effect on rumen microbial flora, and the optimal level for dairy cows is 30 g/d.关键词
茶皂素/奶牛/瘤胃发酵/微生物区系Key words
tea saponin/dairy cow/rumen fermentation/microflora分类
农业科技引用本文复制引用
严淑红,赵士萍,蒋琦晖,方洛云,周敏,闵婉平,蒋林树..茶皂素对奶牛瘤胃发酵及瘤胃微生物区系的影响[J].动物营养学报,2016,28(8):2485-2496,12.基金项目
2014年度北京市教育委员会“北京市属高等学校高层次人才引进与培养计划项目（ CIT＆TCD20130324）”；北京市农业局“北京市现代农业产业技术体系奶牛创新团队” （）
