动物营养学报2016，Vol.28Issue(8)：2512-2520,9.DOI:10.3969/j.issn.1006-267x.2016.08.023
应激和饲粮能量水平对肉仔鸡肝脏脂肪合成能力的影响
Effects of Stress and Dietary Energy Level on Fatty Synthesis in Liver of Broiler Chickens
摘要
Abstract
The objective of this study was to explore fatty acid synthase ( FAS) activity, expressions of AMP⁃activated protein kinase ( AMPK) and sterol⁃regulatory element binding proteins⁃1 ( SREBP⁃1) mRNA in liver of broiler chickens treated by glucocorticoid or dietary energy level in order to explain why stress can raise the hepatic triglyceride and look for ways to relieve stress. There were four trials in this study. In the first and the second trials, broiler chickens fed high and low different energy level of diets were challenged with corticoster⁃one from 3 to 9 days of age and 28 to 34 days of age, respectively. At the end of the two trials, livers were re⁃moved for measuring FAS activity. In the third trial, 108 male Arbor Acres chickens with 7 days of age were divided into 3 groups injected with dexamethasone ( stress group) , saline ( control group) and saline ( a pair⁃fed group, with the same feed intake compared to stress group) for 7 d, respectively. At the 14 days of age, the liver was removed to determine the triglyceride content and expressions of AMPK and SREBP⁃1 mRNA. In the last trial, chickens aged from 3 to 9 days were treated with corticosterone and drinking water supplemented with glucose, and FAS activity was measured in the end. The results showed as follows:1) FAS activity was significantly increased by corticosterone treatment in chickens of 3 to 9 days old (P<0.05), and had the in⁃creasing trend in chickens of 28 to 34 days old ( P=0.051 2) . 2) Compared with the pair⁃fed group, dexam⁃ethasone administration resulted in enhanced triglyceride content in liver ( P<0.05) , and the AMPK mRNA ex⁃pression level in liver was significantly up⁃regulated ( P<0.05) . The SREBP⁃1 mRNA expression level in liver of chickens treated with dexamethasone were significantly higher than those of control and pair⁃fed groups ( P<0.05). 3) Glucose supplementation could significantly decrease the FAS activity in liver of chickens during stress ( P<0.05) . In conclusion, corticosterone treatment can increase the FAS activity in liver of broiler chick⁃ens, dexamethasone administration can increase SREBP⁃1 mRNA expression level in liver and activate AMPK, and glucose can relieve the harm of stress.关键词
肉仔鸡/应激/脂肪沉积/FAS/AMPK/SREBP-1Key words
broiler chickens/stress/lipid deposition/FAS/AMPK/SREBP-1分类
农业科技引用本文复制引用
蔡元丽,林海..应激和饲粮能量水平对肉仔鸡肝脏脂肪合成能力的影响[J].动物营养学报,2016,28(8):2512-2520,9.基金项目
国家自然科学基金 （）
