动物营养学报2016，Vol.28Issue(8)：2521-2533,13.DOI:10.3969/j.issn.1006-267x.2016.08.024
鸭血球短肽的优化制备及其特性研究
Preparation and Characterization of Enzymatic Hydrolysis of Duck Blood Corpuscle Short Peptide
摘要
Abstract
The aims of this study were to screen the commercial protease with high hydrolysis efficiency and good decolorization effect, and to establish process technology of blood corpuscle short peptide, compared the nutritional characteristics before and after enzymatic hydrolysis, to study its nutritional value, functional proper⁃ties and antioxidant activity in vivo. The functional product of blood corpuscle short peptide was produced so as to offer theoretic basis and technical reference for the efficient conversion and utilization of poultry blood. Tak⁃ing degree of hydrolysis ( DH) , decoloration and hydrolysate yield as criterion, the effects of main parameters ( such as types of protease, enzyme dosage, temperature, pH and hydrolysis time) and the optimal hydrolysis conditions were established based on the method of single⁃factor and orthogonal experiments. The blood corpus⁃cle short peptide was used to study its nutritional, functional properties and antioxidant activity. We selected the acidic protease as the best enzyme to hydrolyze protein in our experiments. The suitable conditions of enzymatic hydrolysis, i.e. enzyme dosage 6 000 U/g, hydrolysis temperature 50 ℃, pH 3.5, hydrolysis time 7.0 h, and the degree of hydrolysis was (25.10±0.65)%, hydrolysate yield was (60.09±1.77)% under the optimal con⁃ditions. The molecular weight distribution of the hydrolysate as determined by high performance liquid chroma⁃tography ( HPLC) suggested that significant degradation of blood corpuscle proteins produced the hydrolysate mostly consisting of short peptides below 3 ku with molecules less than 1 ku accounting for the majority ( 62.82%) of the total peptides. The white blood corpuscle short peptide powder containing all the common a⁃mino acids was rich in essential amino acids (53.31%) and had excellent solubility (>60%) and emulsifying stability. With the increasing concentration of blood corpuscle protein, its free radical scavenging activity [1,1⁃diphenyl⁃2⁃picrylhydrazyl radical 2,2⁃diphenyl⁃1⁃(2,4,6⁃trinitrophenyl)hydrazyl (DPPH·) and superoxide anion] and reducing power increased. Therefore, acid protease serves best for the hydrolysis of duck blood cor⁃puscle protein, and its peptide powder has good features with high protein, rich essential amino acids and strong antioxidant activity. The blood corpuscle short peptide can be utilized as functional material for the food and feed industry.关键词
血球短肽/酸性蛋白酶/功能特性/抗氧化活性Key words
blood corpuscle short peptide/acid protease/functional property/antioxidant activity分类
农业科技引用本文复制引用
郑召君,余占桥,卫旭彪,张日俊..鸭血球短肽的优化制备及其特性研究[J].动物营养学报,2016,28(8):2521-2533,13.基金项目
国家“十二五”科技支撑计划（2011BAD26B0403）；农业部科技成果转化资金项目（2011GB2A000009）；国家自然科学基金 （）
