动物营养学报2016，Vol.28Issue(8)：2581-2588,8.DOI:10.3969/j.issn.1006-267x.2016.08.031
基于高通量测定肉鸡回肠微生物多样性及PICRUSt基因预测分析
Analyzing of Ileum Microbial Diversity of Chickens by High-Throughput Sequencing and PICRUSt Predicted
摘要
Abstract
The aim of this study was to analyze the composition and function of ileum microbial community of healthy chickens. The samples of ileum contents of 15 chickens were investigated by high⁃throughput sequen⁃cing. Operational taxonomic units ( OTUs) were picked with a 97% similarity threshold and the sequences less than two in all samples were removed. Than the results were analyzed by bioinformation software and PICRUSt functional profiles. The results showed as follows:1) a total of 424 062 tags were assigned to 1 002 non⁃sin⁃gleton OTUs from all samples. Each sample had (28 271±8 855) tags and (261±82) OTUs on average. 2) In total, 20 phyla and 160 genera were detected, and 4 shared core phyla were contained in all samples. They were Firmicutes (85.35%), Proteobacteria (6.09%), Cyanobacteria (2.15%) and Actinobacteria (0.16%). Nineteen shared core genera were contained in all samples, which consisted 93. 75% of the overall bacteria community. 3) Three hundred and twenty⁃eight functions were detected in all ileum samples. Heat⁃map of the first 20 functions showed that there were some differences in efficiency of each sample. Therefore, our research reveals that the chicken ileum is complex for the phylogenetic diversity of the microbial communities, but the main microbial communities are relatively stable. The findings of this study will provide basic information and lay the foundation of the research in composition and function of the microbiota of chicken ileum.关键词
肉鸡/回肠/高通量测序/核心菌群/PICRUStKey words
chicken/ileum/high-throughput sequencing/core-microbiome/PICRUSt分类
农业科技引用本文复制引用
徐帅,林奕岑,周梦佳,倪学勤,曾东,曾燕..基于高通量测定肉鸡回肠微生物多样性及PICRUSt基因预测分析[J].动物营养学报,2016,28(8):2581-2588,8.基金项目
四川省科技厅国际合作项目 （）
