动物营养学报2016，Vol.28Issue(8)：2599-2606,8.DOI:10.3969/j.issn.1006-267x.2016.08.033
饲粮铜含量对牦牛体外瘤胃发酵的影响
Effects of Dietary Copper Content on Rumen Fermentation of Yaks in Vitro
摘要
Abstract
To find an optimal content of copper in diet for yaks, cupric glycinate was used as additive, and diet for yaks was used as a substrate for in vitro rumen fermentation in the present study. Copper contents in sub⁃strates were designed as 5.0, 10.0, 15.0, 20.0, 25.0 mg/kg, respectively. The fermentation lasted for 48 h. After fermentation, gas production, rumen fermentation parameters and digestive enzyme activities were meas⁃ured. The results showed as follows: when the content of copper in substrate was 15.0 mg/kg, dry matter di⁃gestibility ( DMD) , the concentrations of microbial protein ( MCP) , propionic acid, isobutyric acid, butyric acid, isovaloric acid, valeric acid and total volatile fatty acids, as well as the activities of lipidase, trypsinase , cellulase reached the highest, which were 63. 858 %, 4. 289 g/L, 24. 475 mmol/L, 0. 470 mmol/L, 8.977 mmol/L, 1. 159 mmol/L, 1. 607 mmol/L, 81. 583 mmol/L, 0. 504 U/mL, 84. 167 U/mL and 79.956 U/mL, respectively; when the content of copper in substrate was 10.0 mg/kg, acetic acid/propionic acid reached the lowest, which was 2.045;when the content of copper was 10.0 mg/kg, MCP and acetic acid concentrations reached the highest, which were 4.289 g/L and 51.075 mmol/L, respectively, and the values of other indexes were also on high level. Therefore, under in vitro conditions, the copper content recommended in yak’s diet is between 10.0 and 15.0 mg/kg.关键词
牦牛/甘氨酸铜/体外产气技术/消化酶活力/挥发性脂肪酸Key words
yak/cupric glycinate/in vitro gas production technique/digestive enzyme activity/volatile fatty acid分类
农业科技引用本文复制引用
薛艳锋,郝力壮,刘书杰,柴沙驼,张晓卫,赵索南..饲粮铜含量对牦牛体外瘤胃发酵的影响[J].动物营养学报,2016,28(8):2599-2606,8.基金项目
“973”国家重点基础研究发展计划项目（2012CB722906）；“十二五”国家科技支撑计划项目（2012BAD13B01）；国家国际科技合作专项项目（2015DFG31870）；公益性行业（农业）科研专项（201203008）；教育部长江学者和创新团队发展计划项目（IRT13074）；青海省重大科技平台建设项目 （）
