福建农业学报2016，Vol.31Issue(6)：553-559,7.DOI:10.19303/j.issn.1008-0384.2016.06.001
229份水稻品种及重要育种材料抗稻瘟病 Pik 位点基因型鉴定
Characterization of the Genotypes at the Rice Blast Resistance Pik Locus in 229 Rice Cultivars and Important Breeding Materials
摘要
Abstract
The rice Pik locus that contains multiple blast resistance genes,such as Pi-k ,Pi-km ,Pi-kp ,Pi1 and Pi-kh ,has great utilization value in rice breeding for blast resistance in China.In the present study,we investigated the genotypes at the Pik locus in 229 rice cultivars and important breeding materials.Based on PCR amplification using K-and N-type specific primer sets,80 accessions were identified harboring the K1K2 haplotypes.The 80 K1K2 accessions were further accessed for the presence of Pi-k ,Pi-kp and Pi1 using allele-specific markers in combination with sequencing identification of allele-specific SNPs.Heidao and Jinghu B were identified harboring the Pi-k resistance allele,and Ruanmigu and Enhui 99-64 were identified harboring Pi-kp and Pi1,respectively.In addition,an elite indica restorer line Minhui 3301 was identified harboring the K1K2 haplotypes.Allele-specific marker analysis and sequencing data revealed that the sequence of Minhui 3301 at Pik locus differs from the sequences of Pi-k ,Pi-km ,Pi-kp ,Pi1 and Pi-kh ,suggesting that Minhui 3301 might possess a new allelic gene at the Pik locus.In summary,we validated the distribution of the K1K2 haplotypes at the Pik loucs in 229 rice accessions.Our results provide a wealthy of information for developing and deploying resistant cultivars to control rice blast disease.关键词
水稻/稻瘟病/Pik 位点/闽恢 3301/基因分型Key words
rice/rice blast/Pik locus/Minhui 3301/genotyping分类
生物科学引用本文复制引用
陈子强,田大刚,梁廷敏,陈在杰,胡昌泉,王锋,陈松彪..229份水稻品种及重要育种材料抗稻瘟病 Pik 位点基因型鉴定[J].福建农业学报,2016,31(6):553-559,7.基金项目
福建省自然科学基金杰出青年基金滚动项目（2014J07004）；国家自然科学基金委员会-福建省人民政府促进海峡两岸科技合作联合基金重点支持项目（U1405212）；福建省科技计划项目---省属公益类科研院所基本科研专项 （）
