福建农业学报2016，Vol.31Issue(6)：560-565,6.DOI:10.19303/j.issn.1008-0384.2016.06.002
BDE-47胁迫对双齿围沙蚕抗氧化防御系统的影响
Effect of the Antioxidant Dfense Sstem of Perinereis aibuhitensis Exposed to Tetrabromodiphenyl Ether
摘要
Abstract
Perinereis aibuhitensis is used as biological subject in acute toxicity test,for determining the 96 h-LC50 of tetrabromodiphenyl ether(BDE-47).On this basis,the activities of SOD,CAT ,GST,GPx and the contents of GSH,MDA of the antioxidant defense system of Perinereis aibuhitensis exposed to 0,2,4,8 mg · L-1 of BDE-47 pollutants were determined.The results were as follows:the 96h-LC50 of BDE-47 to Perinereis aibuhitensis was 31.24 mg · L-1 . Compared with the control group,the content of GSH and activities of GST and CAT had basically no significant change, only the GST activity of 8 mg·L-1 group was significantly higher (P <0.01)on 14 th day.The GPx activity of 4 mg· L-1 group was significantly higher (P <0.05)on 1 th day,and 2 mg·L-1 group showed the increasing trend during the 7 th to 14 th day.On 1 th day,the SOD activity of the 4 mg·L-1 and 8 mg·L-1 groups significantly decreased (P <0.05). Overall,during the period of 1-7 d,SOD activity of the 2 mg·L-1 ,4 mg·L-1 groups were induced to rise. On 14 th day,SOD activity in 4 mg·L-1 ,8 mg·L-1 groups were significant (P <0.05)and significantly (P <0.01)inhibited respectively. The MDA content of all the treatment groups were induced to increase (P <0.05),and 4 mg·L-1 group significantly increased (P <0.01)on 1 th day. There was no significant change in the content of MDA of the treatment groups during the exposure period of 4-14 th day,only 8 mg·L-1 group was significantly higher (P <0.05)on 7 th day.In conclusion:the content of MDA and activity of SOD of the antioxidant defense system of Perinereis aibuhitensis had significant changes at the early exposure stage (P <0.05),which could be used as potential biomarkers.关键词
双齿围沙蚕/BDE-47/抗氧化酶/GSH/MDA/生物标志物Key words
Perinereis aibuhitensis/BDE-47/antioxidant enzyme/GSH/MDA/biomarker分类
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宿丽丽,阎希柱..BDE-47胁迫对双齿围沙蚕抗氧化防御系统的影响[J].福建农业学报,2016,31(6):560-565,6.基金项目
国家海洋公益性行业科研专项子课题 （）
