福建畜牧兽医2016，Vol.38Issue(4)：3-5,3.
实时荧光定量RT-PCR法比较猪瘟疫苗病毒含量
Comparison of virus content in swine fever vaccine by fluorogenetic quantitative RT-PCR
摘要
Abstract
To effectively detect the quantity of the viral in swine vaccine in Fujian province, select high effective vaccine for the farms, 23 batches vaccine from 10 different companies collected and used real-time PCR to detect the quantity of the viral. Using standard substance as control, we compared the corresponding RID. The result showed that the percent of pass in 23 batches vaccine is only 69.5%. The quantity of viral in passaged cell vaccine is relative high and the quantity of the viral in spleen vaccine is better than oth-ers. There is a big differences between different companies and between different batches in one company. Real-time PCR is an con-venient and effective method to detect the quantity of the viral in swine vaccine and can provide useful guideline for selecting vaccine.关键词
猪瘟疫苗/荧光定量RT-PCR T/aqMan探针/病毒含量Key words
Swine vaccine/qRT-PCR/TaqMan/Viral load引用本文复制引用
章秋月,马华魁,郑新平,陈鹏强,陈秋勇,胡崇伟,修金生,吴波平..实时荧光定量RT-PCR法比较猪瘟疫苗病毒含量[J].福建畜牧兽医,2016,38(4):3-5,3.基金项目
福建省农业科学技术项目（2014-01）、福建省自然科学基金项目（2013J05042）资助。 （）
