检验医学与临床2016，Vol.13Issue(15)：2100-2102,3.DOI:10.3969/j.issn.1672-9455.2016.15.011
新生儿耐碳青霉烯类肺炎克雷伯菌耐药性及耐药基因研究
Study on drug resistance and drug resistance genes of carbapenem-resistant Klebsiella pneumoniae isolated from neonates
摘要
Abstract
Objective To investigate the antimicrobial resistance and genotypes of carbapenem‐resistant Klebsiella pneumoniae i‐solated from newborn patients .Methods A total of 8 strains of carbapenem‐resistant Klebsiella penemoniae clinically isolated from neonatal inpatients in two maternal and child health care hospitals of Nanning City from December 2013 to December 2015 were col‐lected and identified .The strain identification and minimal inhibitory concentration(MIC) were carried out by using the VITEK au‐tomatic microbiological analyzer .The carbapenemase positive strains were screened by the modified Hodge tests and metallo‐β‐lacta‐mase producing strains by imipenem‐EDTA synergy tests .The carbapenemase genes(KPC ,GES ,SME ,NMC ,IMI ,IMP ,NDM‐1 , VIM ,SIM ) were amplified by polymerase chain reaction (PCR) ,moreover the PCR positive productions were performed the se‐quencing identification .The detection results were performed the comparison with the GenBank database .Results The antibacterial susceptibility test results showed that 8 strains were highly resistant to commonly used antibacterial drugs ,but highly sensitive to fluoroquinolones and aminoglycosides antibacterial drugs .The phenotypic validation test showed that 6 strains were positive in the modified Hodge test and 7 strains were positive in the EDTA synergy test .Eight strains of carbapenem‐resistant Klebsiella peneu‐moniaes were all carried IMP‐4 carbapenemase genes .No KPC ,GES ,SME ,NMC ,IMI ,NDM‐1 ,VIM and SIM types of carbapene‐mase genes were detected .Conclusion Carbapenem‐resistant Klebsiella pneumoniae isolated from neonates in this area has the high resistance to multiple antibacterial drugs ,its main drug resistance mechanism is the production of B‐carbapenemase (B‐MBL) and IM P‐4 is the main enzyme type .关键词
新生儿/肺炎克雷伯菌/耐药性/碳青霉烯类/耐药基因Key words
neonate/Klebsiella pneumoniae/antibacterial resistance/carbapenem/drug resistance genes引用本文复制引用
黄维真,梁军荣,王双杰,张清,唐银琳,黄昌园,黄丽英,潘新年..新生儿耐碳青霉烯类肺炎克雷伯菌耐药性及耐药基因研究[J].检验医学与临床,2016,13(15):2100-2102,3.基金项目
广西医药卫生自筹经费计划课题（Z2014152）。 （）
