摘要
Abstract
Objective To systematically evaluate the accuracy of gene chip technology in identifying isoniazide(INH)‐resistant mycobacterium(M .) tuberculosis by using the meta analysis method .Methods The diagnostic tests of gene chip versus drug sensi‐tive test(gold standard) were retrieved from Cochrane Library ,SCI ,PubMed ,EMbase ,WanFang Data and CNKI by computer .The retrieval time was from the database establishment to May 2015 .Two reviewers independently screened the literatures according to the inclusion and exclusion criteria ,extracted the data ,and assessed the methodological quality of the included studies according to the QUADAS items .The Meta‐DiSc software (version 1 .4) was used to conduct the pooling analysis on sensitivity(SEN) ,specific‐ity(SPE) ,positive likelihood ratio(+ LR) ,negative likelihood ratio(-LR) diagnostic odds ratio(DOR) .The heterogeneity test was performed and the summary receiver operating characteristic (SROC) curve was drawn for calculating the area under the curve (AUC) .Results A total of 15 domestic studies and foreign studies were totally included ,involving 3 967 strains of M .tuberculosis identified by the conventional drug susceptibility test(DST) ,including 1 147 INH‐resistant strains and 2 820 sensitive strains .The SENmerging of INH‐resistant M .tuberculosis detected by gene chip technology was 0 .82[95% CI (0 .79 -0 .84)] ,SPEmerging 0 .97 [95% CI (0 .97-0 .98)] ,+ LR was 21 .81[95% CI (12 .12-39 .25)] ,-LR was 0 .19[95% CI (0 .15-0 .24)] ,DORmerging 136 .55 [95% CI(70 .66-263 .89)] ,AUC was 0 .94 .Conclusion The evidence‐based method systematic evaluation indicates that the gene chip technology has a good diagnostic value in identifying INH‐resistant M .tuberculosis .Compared with the traditional DST ,the gene chip technology is simple operating ,rapidly detect ,which has higher detection rate of drug resistant gene ,but its cost is high‐er .关键词
基因芯片技术/耐药结核分枝杆菌/KatG基因/系统评价/药敏试验Key words
gene chip/drug-resistant M .tuberculosis/KatG gene/systematic evaluation/drug sensitivity test
