检验医学与临床2016，Vol.13Issue(15)：2128-2131,4.DOI:10.3969/j.issn.1672-9455.2016.15.021
上调人1型大麻素受体表达诱导人宫颈癌细胞凋亡的实验研究
Experimental study on up-regulating expression of hCB1R for inducing apoptosis of HeLa cells
张萌 1龙明 2郑慧峰 1杨缙 1李晶2
作者信息
- 1. 重庆市人民医院重症医学科 400014
- 2. 重庆三峡医药高等专科学校 404120
- 折叠
摘要
Abstract
Objective To investigate the effect and mechanism of human type 1 cannabinoid receptor (hCB1R) on the apoptosis of cervical cancer HeLa cell by constructing the gene eukaryotic expression vector .Methods The GV230‐hCB1R plasmid was con‐structed and identified by the double enzyme digestion and DNA sequencing .Then it was transfected into HeLa cells .The expres‐sion and cellular localization(ECL) of hCB1R was detected by Western blot and immunofluorescence cellular chemical staining com‐bined with laser scanning confocal microscope;the apoptosis rate was tested by flow cytometry ;the mRNA and protein expression of hCB1R ,Bcl‐2 ,Bax and Bad was examined by real‐time fluorescent quantitative PCR(qRT‐PCR) .Results After transfection into HeLa cells ,hCB1R protein with the relative molecular mass of 40 000 was expressed in both cytoplasm and cellular membrane of cells .The over expression of hCB1R could up‐regulate the apoptosis of Bax and Bad ,and inhibited the Bcl‐2 expression .The flow cytometry results displayed that HeLa cell line showed apoptosis ,hCBIR had obvious inhibiting effect on the growth of cervical cancer HeLa cell line and promoted apoptosis .Conclusion Up‐regulating expression of hCB1R in Hela cells could enhance the ex‐pression of Bax and Bad inhibit the expression of Bcl‐2 ,thus inducing the cellular apoptosis .关键词
人1型大麻素受体/HEK293细胞/宫颈癌细胞/细胞凋亡Key words
human type 1 cannabinoid receptor/HEK293 cell/cervical cancer cell/apoptosis引用本文复制引用
张萌,龙明,郑慧峰,杨缙,李晶..上调人1型大麻素受体表达诱导人宫颈癌细胞凋亡的实验研究[J].检验医学与临床,2016,13(15):2128-2131,4.
