农药学学报2016，Vol.18Issue(4)：465-471,7.DOI:10.16801/j.issn.1008-7303.2016.0065
植物内生枯草芽孢杆菌 Em7菌株对葡萄灰霉病菌的抑菌活性
Antifungal activity of plant endophyticBacillus subtilis strain Em7 againstBotrytis cinerea
摘要
Abstract
The inhibition effect of plant endophyteBacillus subtilis strain Em7, isolated from wheat roots, againstBotrytis cinerea Pers. was studied, by using the antifungal test on medium plates, inhibition test of conidia germination,in vitro inoculation on fruit, and observation with electron microscopy. Confronting incubation ofB. subtilis Em7 andB. cinerea on PDA medium resulted in obvious inhibition zones, with the diameter of 2.81 cm. Em7 treatment led to 88.65% failure germination of theB. cinerea conidia.In vitro inoculation assay verified that Em7 treatedB. cinerea exhibiting reduced pathogenicity on grape fruit with a lower disease index, and the relative control effect reached 78.92% compared to the controls. Moreover, scanning electron microscopy observation revealed a malformed morphology of the Em7 treated fungus mycelium, with an uneven surface, a local swelling node or constriction, more branches, increased vacuoles, thicken cell wall, and altered cell membrane permeability. The plant endophyticB. subtilis Em7 can effectively inhibitB. cinerea and reduce the fungus pathogenicity.关键词
葡萄灰霉病菌/枯草芽孢杆菌/抑菌作用/超微结构Key words
Botrytis cinerea Pers./Bacillus subtilis/inhibitory effects/sultrastructure分类
资源环境引用本文复制引用
伏波,姚娟妮,高小宁,黄丽丽,康振生,韩青梅..植物内生枯草芽孢杆菌 Em7菌株对葡萄灰霉病菌的抑菌活性[J].农药学学报,2016,18(4):465-471,7.基金项目
陕西省科技统筹计划课题(2013KTDZ02-01-03)；高等学校学科创新引智项目(B07049) （2013KTDZ02-01-03）
