热带亚热带植物学报2016，Vol.24Issue(4)：456-463,8.DOI:10.11926/j.issn.1005-3395.2016.04.014
柑桔叶点霉侵染琯溪蜜柚的超微结构观察及致病机理分析
Destruction Characteristics of Hypodermal Cells inCitrus grandis ‘Guanximiyou’ Infected byPhyllosticta citriasiana and Pathogenic Mechanism
摘要
Abstract
In order to explore the characteristics of infection and damage of citrus black spot toCitrus grandis ‘Guanximiyou’, the changes in hypodermal cell structure were observed under scanning electron microscopy and transmission electron microscopy (TEM) after artificially inoculated withPhyllosticta citriasiana. After pomelo fruits were infected, the tonoplast in hypodermal cell wrinkled, fractured, and eventually vanished. The starch grains in chloroplast disappeared. The plasmolemma wrinkled, invaginated and partly ruptured. The number of mitochondria increased with clear visible grana. The thylakoid swelled and disintegrated. Afterwards, mitochondria underwent morphological changes, including disappearance of two layers of membrane and vacuolization. However, there were no intercellular or intracellular hyphae, and the cell wall remained structurally stable.Phyllosticta citriasiana infected hypodermal cells of ‘Guanximiyou’ through toxins, which probably attack cell membrane structure and cross over cell wall through plasmodesmata.关键词
琯溪蜜柚/黑斑病/侵染/超微结构/致病机理Key words
Guanxi pomelo/Citrus black spot/Infection/Ultrastructure/Pathogenic mechanism引用本文复制引用
牛先前,罗金水,卢松茂,杜丽君,郑国华,林秀香..柑桔叶点霉侵染琯溪蜜柚的超微结构观察及致病机理分析[J].热带亚热带植物学报,2016,24(4):456-463,8.基金项目
国家科技支撑计划项目(2014BAD15B00) （2014BAD15B00）
福建省公益类基本科研专项(2009R10010-1)资助This work was supported by the National Science and Technology Support Project (Grant No.2014BAD15B00), and the Special Project for Public Basic Scientific Research of Fujian Province (Grant No.2009R10010-1) （2009R10010-1）
