日用化学工业2016，Vol.46Issue(7)：377-381,426,6.DOI:10.13218/j.cnki.csdc.2016.07.003
响应面法优化月桂酸单甘油酯的酶催化合成工艺
Process optimization for enzymatic synthesis of monolaurin using response surface methodology
摘要
Abstract
Monolaurin was prepared by glycerolysis of trilaurin with lipase as the catalyst and t - butanol as solvent. Optimization of the reaction process was conducted based upon single factor tests and adopting response surface methodology. The results showed that water content (mass fraction referring to glycerol)is the most significant factor on the yield of monolaurin. The suitable conditions are as follows:rotation speed of the oscillator,100 r/ min;enzyme dosage,5% (based on the amount of total substrates,trilaurin and glycerol);molar ratio (glycerol to trilaurin),6 ∶ 1;mass fraction of the total substrates,51. 9% (referring to the total amount of all reactants and solvent);reaction temperature,65 ℃;water content,4. 23%;and reaction time, 5 h. The experimental yield of monolaurin achieves 81. 32% under the aforesaid conditions,which is well in conformity with the predicted value of 81. 68% . Therefore,the regression model shows good predictability.关键词
月桂酸单甘油酯/甘油解反应/响应面分析法/酶催化合成Key words
monolaurin/glycerolysis/response surface methodology/enzymatic synthesis分类
化学化工引用本文复制引用
邓成龙,张桂菊,吴望波,王瑞,徐宝财,珠峰..响应面法优化月桂酸单甘油酯的酶催化合成工艺[J].日用化学工业,2016,46(7):377-381,426,6.基金项目
“十二五”国家科技支撑计划资助项目（2014BAE03B01）；国家自然科学基金资助项目（21403010）；北京市教委科技计划重点资助项目（KZ201510011010）；科技成果转化－提升计划资助项目 （）
