世界中医药2016，Vol.11Issue(7)：1145-1149,5.DOI:10.3969/j.issn.1673-7202.2016.07.002
夏枯草抗疱疹病毒多糖的提取及纯化工艺研究
Extraction and Purification of Anti-HSV Polysaccharides from Prunella Vulgaris
摘要
Abstract
Objective:The aim of this study is to optimize the extraction and purification process for anti-HSV polysaccharides from Prunella Vulgaris. Methods:The extraction method was modified by an orthogonal array experiment on three variable parameters:extraction time,solvent volume and extraction times. The content of polysaccharides and extraction yield were used as index. The effects of ultrafiltration technology and alcohol precipitation were compared in the purity of polysaccharide and anti-HSV activity. Results:The optimized extraction parameters are three extractions,1. 5 hour extraction time,14 times of water for the first extraction and 12 times of water for the second and third extraction. The polysaccharide obtained from ultrafiltration technology and alcohol precipitation showed anti-HSV-1 activity in MTT assay,with IC50 values of(93. 99 ± 13. 12)μg/mL and(51. 35 ± 15. 30)μg/mL,respectively. The results suggested that the two purification processes have significantly different degrees of polysaccharide puri-ty. The anti-HSV activity of polysaccharide from ultrafiltration technology was significantly higher than that of alcohol precipitation. Conclusion:The polysaccharides from Prunella Vulgaris obtained using ultrafiltration technology have higher anti-HSV activity than that isolated by alcohol precipitation.关键词
夏枯草/抗疱疹病毒/多糖/提取/纯化/醇沉/超滤Key words
Prunella Vulgaris L./Anti-HSV/Polysaccharides/Extraction/Purification/Alcohol precipitation/Ultrafiltration分类
医药卫生引用本文复制引用
孔思远,吴蓉,蔡双璠,谭红胜..夏枯草抗疱疹病毒多糖的提取及纯化工艺研究[J].世界中医药,2016,11(7):1145-1149,5.基金项目
“重大新药创制”科技重大专项“十二五”计划(编号:2013ZX09103002-020) （编号:2013ZX09103002-020）
