世界中医药2016，Vol.11Issue(7)：1337-1339,3.DOI:10.3969/j.issn.1673-7202.2016.07.049
正交设计法优选忍冬藤中绿原酸提取工艺
Optimization of Extraction Process of Chlorogenic Acid in Lonicerae japonicae Caulis by Orthogonal Design
摘要
Abstract
Objective:To study the extraction methods for chlorogenic acid in Lonicerae japonicae Caulis. Methods:The micro-wave extraction and ultrasonic extraction of chlorogenic acid in Lonicerae japonicae Caulis were set based on orthogonal design. The chlorogenic acid contents of different extraction techniques were detected by HPLC. The results of the two extraction methods were compared. Results:The results show that the chlorogenic acid was highest, up to 0. 357%, when microwave time was set at 60s with 50% ethanol concentration and solid-liquid ratio of 1∶15 by microwave extraction. The chlorogenic acid was highest when methanol concentration, up to 0. 321%, was set at 50%, ultrasonic power 50W and solid-liquid ratio 1:20 by ultrasonic extrac-tion. By taking chlorogenic acid content as an indicator, microwave extraction method was better than ultrasonic extraction. Con-clusion:According to the results, the best extraction method for Chlorogenic acid in Lonicerae japonicae Caulis was microwave ex-traction with less time cost and low energy consumption and the extraction rate is better than that of the ultrasonic extraction. It can be applied in the extraction of chlorogenic acid in Lonicerae japonicae Caulis.关键词
忍冬藤/绿原酸/正交设计/提取方法/含量测定Key words
Lonicerae japonicae Caulis/Chlorogenic acid/Orthogonal design/Extraction methods/Determination分类
医药卫生引用本文复制引用
毛莹,李东霞,夏春丽..正交设计法优选忍冬藤中绿原酸提取工艺[J].世界中医药,2016,11(7):1337-1339,3.基金项目
辽宁省大连市科技计划项目(编号:2008E11SF171) （编号:2008E11SF171）
