局解手术学杂志2016，Vol.25Issue(8)：551-554,4.DOI:10.11659/jjssx.04E016071
Mrh-aFGF对帕金森病大鼠中脑腹侧被盖区神经元的保护
Protective effect of Mrh-aFGF on the neurons in ventral tegmental area of rats with Parkinson’ s disease
摘要
Abstract
Objepctive To explore the protective effect of modified recombinant human aFGF ( Mrh-aFGF) on the neurons in ventral tegmental area of rats with Parkinson’ s disease ( PD) . Methods The 54 SD rats were ramdomly divided into the control group,the model group and the treatment group,and there were 18 rats in each group. PD rats of the model group and the treatment group were induced by in-jecting 6-OHDA into the left substantia nigra compacta ( SNC) and ventral tegmental area ( VTA) to build the PD model. Rats in the treat-ment group were given Mrh-aFGF injection after lateral ventricle injection,and the behavioral changes of the rats were detected after apomor-phine injection. The morphologic features and pathological changes of neurons in the ventral tegmental area were observed by Nissl’ s staining and electronic microscope. Results Compared to the right VTA of PD rats,the number of neurons in left side ( the injured side) decreased significantly in the model group(P<0. 05). In the treatment group,the structure of left (the injured side) VTA was markedly improved and the number of neurons was increased one week,two weeks and four weeks after operation compared with the model group (P<0. 05). The neurons in the VTA of the model group were found to have karyopyknosis,endoplasmic reticulum,degranulation,mitochondria swelling,cristae disappear,pre-synaptic and post-synaptic membranes swelling,and synaptic cleft disappear. In the treatment group,the ultrastructure of the neurons in the VTA,such as nuclei,mitochondria,synaptic structure,kept well compared to the model group. Conclusion Mrh-aFGF could protect the neurons in the ventral tegmental area from the loss and improve the ultrastructure of the neurons of PD rats.关键词
帕金森病/重组人改构体酸性成纤维细胞生长因子/中脑腹侧被盖区/超微结构/大鼠Key words
Parkinson’ s disease/modified recombinant human acid fibroblast growth factor/ventral tegmental area/ultrastructure/rat分类
医药卫生引用本文复制引用
肖春苟,蒋威,李力强,邹锦慧,沈伟哉..Mrh-aFGF对帕金森病大鼠中脑腹侧被盖区神经元的保护[J].局解手术学杂志,2016,25(8):551-554,4.基金项目
广东省肇庆市科技创新指导类项目(00188651150605028) （00188651150605028）
