局解手术学杂志2016，Vol.25Issue(8)：555-557,558,4.DOI:10.11659/jjssx.03E016063
致死剂量微波辐照后小鼠肝超微结构及HSP70表达的变化
Ultrastructural changes and HSP70 expression in mice liver after microwave irradiation with lethal dose
王小华 1余争平 2刘明树 1刘淑芳 1黄映雪 1王瑞春 1江伟 1杨灶 1齐宏基 1周舟2
作者信息
- 1. 重庆市公安局渝中区分局物证鉴定所，重庆400013
- 2. 第三军医大学劳动卫生学教研室，教育部电磁生物效应重点实验室，重庆400038
- 折叠
摘要
Abstract
Astract:Objective To investigate the ultrastructural changes and HSP70 expression in liver of mice after microwave irradiation with lethal dose and to explore the application of these indexes as the basis of medical identification in microwave irradiation induced death. Methods The mice were divided into the control group and the irradiation group. Mice of the irradiation group were induced death by whole body exposure to 129 W/cm2 microwave irradiation for 30 minutes. The ultrastructure of liver was observed by transmission electron micro-scope;changes of the HSP70 mRNA and protein expression in liver were detected by reverse transcription polymerase chain reaction ( RT-PCR) and Western blotting respectively. Results Liver cytoplasm was observed dissolved with points and sheets and there were mitochondri-al crest and membrane solution in the irradiation group. And the HSP70 mRNA and protein expression level increases significantly compared with the control group with statistically significant difference (P<0. 01). Conclusion Death induced by microwave irradiation could lead to liver cytoplasm dissolution, mitochondria damage, mRNA and protein expression of HSP70 up-regulation, which may be used as important diagnostic indicators of microwave irradiation induced death.关键词
微波/致死剂量/肝/HSP70Key words
microwave/lethal dose/liver/HSP70分类
医药卫生引用本文复制引用
王小华,余争平,刘明树,刘淑芳,黄映雪,王瑞春,江伟,杨灶,齐宏基,周舟..致死剂量微波辐照后小鼠肝超微结构及HSP70表达的变化[J].局解手术学杂志,2016,25(8):555-557,558,4.
