中国临床医学2016，Vol.23Issue(3)：261-264,4.
组蛋白甲基化抑制剂DZNep对小鼠缺血再灌注损伤肾脏的保护作用
Protective effect of histone methylation inhibitor DZNep on kindey in ischemia reperfusion injury of kidney transplantation in mice
李佳蔚 1李龙 1张潮 1王继纳 1戎瑞明1
作者信息
- 1. 复旦大学附属中山医院泌尿外科，上海 200032
- 折叠
摘要
Abstract
Objective:To study the protective effect of histone methylation inhibitor DZNep on kidney at early stage in the model of renal ischemia reperfusion injury in mice .Methods:18 C57BL/6 mice were randomly divided into sham operation group (sham group) ,ischemia reperfusion injury group (IR group) and ischemia reperfusion plus therapy group (IR+DZNep group) .The model of ischemia reperfusion injury was established in the latter two groups .The IR+DZNep group was given bilateral inguinal subcutaneous injection of DZNep (100 μL per mg/kg) .Bilateral renal pedicles were isolated but not clamped . Renal function and histopathological changes were evaluated 36 h after operation .The apoptosis of renal tubular epithelial cells was detected by TDT-mediated dUTP nick end labeling (TUNEL) assay .Infiltration of inflammatory cells was evaluated through the detection of myeloperoxidase (MPO) .Inflammatory cytokine levels in renal tissue were detected by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) .Results:After DZNep treatment ,the serum creatinine and urea nitrogen level decreased when compared with IR group (69 .17 ± 3 .49 vs 103 .83 ± 14 .62 ,9 .56 ± 1 .07 vs 30 .75 ± 15 .83 ;P<0 .05) .Apoptotic renal cells in DZNep group also declined than IR group(P< 0 .05) .Conclusions:DZNep can reduce the degree of renal ischemia reperfusion injury in mice by inhibiting cell apoptosis and reducing inflammatory reaction , and play a role in protecting the kidney .关键词
DZNep/表观遗传修饰/肾脏/缺血再灌注损伤Key words
DZNep/epigenetic modification/kidney/ischemia reperfusion injury分类
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李佳蔚,李龙,张潮,王继纳,戎瑞明..组蛋白甲基化抑制剂DZNep对小鼠缺血再灌注损伤肾脏的保护作用[J].中国临床医学,2016,23(3):261-264,4.
