中国临床医学2016，Vol.23Issue(3)：265-268,4.
中华眼镜蛇毒口服和静脉注射单次给药对小鼠毒性作用的比较
The acute toxicity of Najanajaatra venom (NNAV) by oral and intravenous administration
张甜 1薛洁 1王响英 2朱路佳 1孙志红 3秦正红1
作者信息
- 1. 苏州大学医学部药学院，苏州 215000
- 2. 苏州大学基础医学与生物科学学院电镜室，苏州 215000
- 3. 苏州大学基础医学与生物科学学院法医系，苏州 215000
- 折叠
摘要
Abstract
Objective:To compare the lethal dose 50 (LD50 ) of Najanajaatra venom (NNAV) with two routes of drug administration and to explore the target organs of acute toxicity of NNAV ,and to observe the acute poisoning symptoms of the mice and organ lesions with oral and intravenous administration of NNAV . Methods: Two different modes of drug administration (oral administration and intravenous injection) were used to determine the LD50 .Within 14 of survival time period ,the activities ,signs and death of the mice were observed .Then ,pathology analysis was applied to the main organs of the dead and survival mice .Results:The LD50 of NNAV by oral administration and intravenous injection was 102 .3 and 0 .624 mg/kg ,and the 95% confidence limit range was 84 .9-123 .3 mg/kg and 572 .3-680 μg/kg ,respectively .The poisoning does by oral administration was 100 times larger than that by intravenous injection ,indicating NNAV could be absorbed through gut . Conclusions:The toxicity of NNAV by intravenous administration is larger than that by oral administration .The death caused by acute intoxication is mainly due to respiratory failure .The organ damage caused by acute intoxication mainly occurred in lung and liver and it was more sever by intravenous injection .关键词
中华眼镜蛇毒/急性毒性试验/小鼠/呼吸衰竭Key words
NNAV/acute toxicity/mice/respiratory failure分类
医药卫生引用本文复制引用
张甜,薛洁,王响英,朱路佳,孙志红,秦正红..中华眼镜蛇毒口服和静脉注射单次给药对小鼠毒性作用的比较[J].中国临床医学,2016,23(3):265-268,4.
