中国临床医学2016，Vol.23Issue(3)：399-401,3.
慢病毒介导2型阿诺碱受体RN A干扰有效靶序列的筛选
Screening of effective target sequence of lentivirus-mediated RyR-2 RNA interference
摘要
Abstract
Objective: To screen the effective target sequence of lentivirus-mediated RyR-2 RNA interference . Methods:We designed five RyR-2 interference candidate target sequences ,cloned them into lentiviral vectors ,packaged into lentivirus in HEK-293T cells , and infected cardiomyocytes . We identified the effeciency of target sequences by real-time fluorescence quantitative PCR and Western blotting .Results:We got 5 RyR-2 interference target sequences through databases , and successfully cloned them into lentivirus vectors ,and later harvested lentivirus and infected cardiomyocytes .Real-time PCR and Western blotting results showed that RyR-2 in cardiomyocytes was downregulated significantly by shRyR-2_1007 (P<0 .05) .Conclusions:Lentivirus-mediated RyR-2 RNA interference could downregulate RyR-2 in cardiomyocytes .关键词
2型阿诺碱受体/慢病毒/RNA干扰Key words
RyR-2/Lentivirus/RNA interference分类
医药卫生引用本文复制引用
杨春杰,丁志文,龚惠,邹云增..慢病毒介导2型阿诺碱受体RN A干扰有效靶序列的筛选[J].中国临床医学,2016,23(3):399-401,3.基金项目
国家自然科学基金（81200085）．Supported by National Natural Science Foundation of China（81200085）． （）
