中国全科医学2016，Vol.19Issue(23)：2798-2801,4.DOI:10.3969/J.issn.1007-9572.2016.23.012
24小时快速眼动睡眠剥夺对青少年期 C57BL/6J 小鼠空间学习记忆能力和海马一氧化氮水平的影响研究
Effect of 24 h Rapid Eye Movement Sleep Deprivation on the Spatial Learning and Memory Ability and Nitric Oxide Level of Hippocampus in Adolescent C57BL/6J Mice
摘要
Abstract
Objective To explore the effect of 24 h rapid eye movement sleep deprivation( REMSD)on spatial learning and memory ability and nitric oxide( NO)level of hippocampus in adolescent C57BL/ 6J mice. Methods From January 2014 to January 2015,45 adolescent male C57BL/ 6J mice(3 - 4 weeks old)at SPF level were selected. Random number table method was used to divide the mice into control group,wide platform(WP)group and REMSD group with 15 in each group. Mice in REMSD group were put into sleep deprivation water tank to establish REMSD model,while mice in WP group were put into artificial water tank to establish REMS fake deprivation model. Three - round place navigation of Morris water maze was applied to test the learning ability and record the escape latent period;spatial probe test of Morris water maze was used to test the memory ability of mice,record times of mice crossing platforms,standing time and the moving distance in the target quadrant. Mice were sacrificed by cervical dislocation to separate hippocampus and test NO level by using NO detection kit. Results Mice in REMSD group presented hyperactivity,wet and messy hair with poor glossiness,limbs congestion,agitated activity,irritability and enhanced excitability. There was significant difference in the escape latent period among mice in each group in the three - round Morris maze place navigation test( P < 0. 05);the escape latent period of REMSD group was significantly shorter than that of control group and WP group(P < 0. 05). There was significant difference in the times of mice crossing platforms in each group(P < 0. 05);the times of mice crossing platforms in REMSD group was significantly more than that of control group and WP group(P < 0. 05). NO level of hippocampus of control group,WP group and REMSD group was (43. 6 ± 8. 5), (45. 8 ± 9. 1),and(54. 2 ± 5. 8)μmol/ L respectively,showing significant differences( F = 7. 460,P= 0. 002);NO level in hippocampus of mice in REMSD group was significantly higher than that of control group and WP group (P < 0. 05). Conclusion The ability of learning and memorization of mice in adolescent period has improved after 24 h REMSD,and this may be related with the increasing NO level of hippocampus.关键词
睡眠,快速眼运动/青少年/学习/记忆/一氧化氮Key words
Sleep,rapid eye movement/Adolescent/Learning/Memory/Nitric oxide分类
医药卫生引用本文复制引用
郝彦利,黄鑫焱,THOIDINGJAM Bidyarani Chanu,张斌..24小时快速眼动睡眠剥夺对青少年期 C57BL/6J 小鼠空间学习记忆能力和海马一氧化氮水平的影响研究[J].中国全科医学,2016,19(23):2798-2801,4.基金项目
广东省科技厅社会发展领域科技计划项目 （）
