中国全科医学2016，Vol.19Issue(23)：2802-2809,8.DOI:10.3969/J.issn.1007-9572.2016.23.013
α7烟碱型乙酰胆碱受体基因敲除对非酒精性脂肪性肝炎小鼠肝脏炎症的影响研究
Effect of α7nAChR Gene Knockout on the Liver Inflammation of Non-alcoholic Steatohepatitis Mice
摘要
Abstract
Objective To investigate the effect of α7nAChR gene knockout on the liver inflammation of non - alcoholic steatohepatitis(NASH)mice. Methods From June 2014 to May 2015,40 SPF male 6 - week - old C57BL/ 6J mice and 40 SPF male 6 - week - old α7nAChR gene knockout mice were selected. The mice were divided into C57 mice normal diet group (group 1),C57 mice high fat and fructose diet group( group 2),gene knockout mice normal diet group( group 3),gene knockout mice high fat and fructose diet group(group 4),with 20 rats in each group. Group 1 and group 3 were fed up with either normal feed,group 2 and group 4 were fed up with high - fat feed plus 20% fructose drinking water,24 weeks to generate an NASH model. The body weight of the mice during the feeding period was measured on a regular basis,and blood was sampled from the caudal vein to detect the levels of ALT,AST,TG and TC. In week 21,group 2 and group 4 were given 400 μg/ kg nicotine by intraperitoneal inJection,and group 1 and group 3 were given the same volume of 0. 9% sodium chloride solution by intraperitoneal inJection for one time per day for 3 weeks. 24 weeks later,the mice were killed. Blood was sampled from eyeball to detect the levels of IL-6 and TNF-α by ELISA method,the changes of color and liver wet weight were recorded,and liver pathological section staining was conducted to observe inflammation degree and fatty degeneration. Results In week 12,15, 18,21 and 24,group 2 and group 4 were higher than group 1 and group 3 in body weight(P < 0. 05). In week 12,15,18, 21 and 24,group 4 was higher than group 2 in body weight(P < 0. 05). Group 2 and group 4 had higher ALT,AST,TG and TC levels in week 8,16 and 24 and higher IL-6 and TNF-α levels and higher liver wet weight in week 24 than group 1 and group 3(P < 0. 05);group 4 had higher ALT and AST levels in week 8,16 and 24,higher TG and TC levels in week 16 and 24, higher IL-6 and TNF-α levels and higher liver wet weight in week 24 than group 2(P < 0. 05). The pathological section of HE and oil red staining showed that group 1 and group 3 had no obvious inflammation degree and fatty degeneration,and group 2 and group 4 showed slight inflammation and fatty degeneration which was mainly manifested as small fat drops in week 8 and showed severer inflammation and fatty degeneration which featured medium or large small fat drops in week 16 and 24,and group 4 had severer inflammation degree and fatty degeneration than group 2. Conclusion α7nAChR gene knockout can significantly aggravate the liver inflammation in NASH mice.关键词
非酒精性脂肪性肝炎/基因敲除技术/α7 烟碱型乙酰胆碱受体/细胞因子类/炎症Key words
Non - alcoholic steatohepatitis/Gene knockout techniques/α7nAChR/Cytokines/Inflammation分类
医药卫生引用本文复制引用
李富强,吴小翠,徐丽娜,陈小梅,唐翠兰..α7烟碱型乙酰胆碱受体基因敲除对非酒精性脂肪性肝炎小鼠肝脏炎症的影响研究[J].中国全科医学,2016,19(23):2802-2809,8.基金项目
国家自然科学基金资助项目（81100279）；浙江省新苗人才计划项目 （）
