中国全科医学2016，Vol.19Issue(24)：2908-2915,8.DOI:10.3969/j.issn.1007-9572.2016.24.008
Neuritin 对大鼠蛛网膜下腔出血后早期脑损伤影响的研究
Effects of Neuritin in Early Brain Injury After Subarachnoid Hemorrhage of Rats
摘要
Abstract
Objective To investigate the effects of Neuritin in endoplasmic reticulum stress factor - GRP78 and blood- brain barrier permeability during the process of early brain injury ( EBI) after subarachnoid hemorrhage ( SAH) of rats. Methods 144 healthy adult male SD rats were selected from April 2014 to September 2015,and they were divided into control group,Sham group,SAH group and Neuritin group with 36 rats in each group,according to random number table method. Then according to simple random number table method,6 rats were selected to be executed from each group at time points of 3,6,12,24,48,72 h respectively and lateral ventricle injection with Neuritin was given to rates in Neuritin group by stereotactic technique 1 h before execution. 6,12,24,48,72 h after modeling,Garcia neurological score of rats in each group was recorded before their execution;3,6,12,24,48,72 h after modeling,HE staining was used to observe the SAH establishment situation,Meilan was microinjected into lateral ventricles to identify Neuritin injection condition. we detected the protein expression of endoplasmic reticulum stress factor GRP78 in cerebral cortex of rats by Western blotting method,and the Evans blue ( EB ) content in brain of rats by formamide immersion method to evaluate the blood - brain barrier permeability. Results 6,12,24,48,72 h after modeling,Garcia neurological scores of rats in SAH group and Neuritin group were lower than those of control group and Sham group( P ﹤ 0. 05);6,12,24,48,72 h after modeling,Garcia neurological score of rats in Neuritin group was higher than that of SAH group( P ﹤ 0. 05). Hematocele was scattered in the surface of brain of rats in SAH group,and there also was large amount of hematocele in willis ring,cerebellar medulla and ventral brainstem. Meilan circulated around the ventral side of the brain of rats in Neuritin group,while no Meilan in the reverse side of the brain. Intracerebroventricular injection of rats in Neutritin group was succeeded. 3,6,12,24,48,72 h after modeling,GRP78 expression level of rats in SAH group was higher than that of control group and Sham group(P ﹤ 0. 05);6, 12,24,48,72 h after modeling,GRP78 expression level of rats in Neuritin group was lower than that of SAH group( P ﹤0. 05). 3,6,12,24,48,72 h after modeling,EB content of brain tissue of rats in SAH group was higher than that of control group and Sham group(P ﹤ 0. 05);3,12 h after modeling,EB content of brain tissue of rats in Neuritin group was higher than that of SAH group(P ﹤ 0. 05),6,48,72 h after modeling,EB content of brain tissue of rats in Neuritin group was lower than that of SAH group(P ﹤ 0. 05). Conclusion There are endoplasmic reticulum stress responses in the process of EBI after SAH. Neurological factor Neuritin can significantly change the protein expression of endoplasmic reticulum stress factor GRP78,improve the neurobehavioral scores of rates in EBI process,and influence the blood - brain barrier permeability.关键词
蛛网膜下腔出血/脑损伤/神经生长因子类/血脑屏障/内质网应激/Neuritin/GRP78Key words
Subarachnoid hemorrhage/Brain injury/Nerve growth factors/Blood - brain barrier/Endoplasmic reticulum stress/Neuritin/GRP78分类
医药卫生引用本文复制引用
李晓天,赵冬,代林志,朱立仓,田卫东,唐仕军,朱文学,杨鹏,王业忠..Neuritin 对大鼠蛛网膜下腔出血后早期脑损伤影响的研究[J].中国全科医学,2016,19(24):2908-2915,8.基金项目
国家自然科学资金资助项目 （）
