中国全科医学2016，Vol.19Issue(24)：2915-2919,5.DOI:10.3969/j.issn.1007-9572.2016.24.009
核转录因子κBp65和骨桥蛋白在子宫内膜异位症中的表达及相关性研究
Expression of NF-κB p65 and OPN in Endometriosis and Their Correlations Research
摘要
Abstract
Background The etiology and pathogenesis of endometriosis( EMs) is still unclear with limited therapeutic measures,which seriously influence the physical and mental health of women. Objective To investigate the expression of NF-κB p65 and osteopontin(OPN)in EMs tissues and their correlations. Methods 35 patients with EMs,who had been implemented operation in Department of Gynaecology and Obstetrics in General Hospital of Ningxia Medical University from January to December 2013,were selected as the case group,of which 17 are in proliferative phase and 18 in secretory phase;30 cases with normal endometrium,who had taken contraceptive operation at the same period and were diagnosed without EMs,were enrolled as a comparison( normal endometrium group),15 cases are in proliferative phase and 15 in secretory phase. Immunohistochemistry SP method and RT-PCR were applied to detect the expression of NF-κB p65,OPN and their mRNA in normal endometrium group,eutopic endometrium and ectopic endometrium groups respectively. Results NF-κB p65 and OPN were mainly expressed in the cytoplasm of endometrial glandular epithelial cells and few were expressed in stromal cells. The expression of NF-κB p65 and OPN in EMs eutopic and ectopic endometrium groups of EMs eutopic and ectopic endometrium groups were higher than that in normal endometrium group,their expression in EMs ectopic endometrium groups was higher than that in EMs eutopic endometrium group(P ﹤ 0. 05). There was no significant difference in the expression of NF-κB p65 proteins in proliferative phase and secretory phase within the three groups(P ﹥ 0. 05),the expression of OPN in secretory phase of EMs eutopic and ectopic endometrium groups were higher than that in proliferative phase(P ﹤ 0. 05);the expression of NF-κB p65 and OPN showed a positive correlation in EMs eutopic and EMs ectopic endometrium groups(rb = 0. 88 and rc = 0. 78,P ﹤ 0. 05) . The relative expression of NF-κB p65,OPN mRNA in EMs eutopic and EMs ectopic endometrium groups was higher than that in normal endometrium group,and their expression in EMs ectopic endometrium group was higher than that in EMs eutopic endometrium group(P ﹤ 0. 05). Conclusion The expression of NF-κB p65,OPN and their mRNA in eutopic and ectopic endometrial tissues increases obviously and they are positively correlated with each other,which indicates that the mutual activation of them may induce the occurrence and development of EMs.关键词
子宫内膜异位症/核因子 κB/骨桥蛋白质Key words
Endometriosis/NF-kappa B/Osteopontin分类
医药卫生引用本文复制引用
白治苗,李望舒,姚卫卫,卢玉凤,杨眉,哈春芳..核转录因子κBp65和骨桥蛋白在子宫内膜异位症中的表达及相关性研究[J].中国全科医学,2016,19(24):2915-2919,5.基金项目
国家自然科学基金资助项目（81160078）；2015 生殖与遗传基础及临床研究创新团队开放课题 （）
