中国全科医学2016，Vol.19Issue(24)：2925-2929,5.DOI:10.3969/j.issn.1007-9572.2016.24.011
米非司酮对子宫内膜异位症子宫内膜腺上皮细胞骨桥蛋白和基质金属蛋白酶9表达的影响
Influence of Mifepristone on the Expression of Osteopontin and Matrix Metalloproteinase 9 in the Endometrium Epithelial Cells of Patients With Endometriosis
杨眉 1蒋春樊 2哈春芳3
作者信息
- 1. 441021 湖北省襄阳市中心医院湖北文理学院附属医院妇产科
- 2. 宁夏医科大学
- 3. 441021 湖北省襄阳市中心医院湖北文理学院附属医院病理科
- 折叠
摘要
Abstract
Background The therapeutic method towards endometriosis is still in limitation,and mifepristone can be used to treat endometriosis in clinic,but its specific mechanism still remains unclear. Objective To investigate the influence of miferpristone on the expression of osteopontin(OPN)and matrix metalloproteinase 9(MMP - 9)in the endometrium epithelial cells of patients with endometriosis. Methods 21 patients with endometriosis,who suffered ovarian cyst enucleation or adnexectomy due to ovarian chocolate cyst in Department of Gynecology in General Hospital of Ningxia Medical University from August 2014 to March 2015,were recruited in the study. They were divided into mifepristone group(11 cases)and control group (10 cases)based on whether they had undergone mifepristone therapy. The eutopic and ectopic endometrium of all these patients were obtained through surgery,and immunohistochemistry method was used to detect the expression level of OPN and MMP - 9;the culture of endometrium epithelial cells in the eutopic endometrium of patients in control group were performed with the intervention of different doses of mifepristone(0,0. 1,1. 0,10. 0 mmol/ L),and inverse transcription - polymerase chain reaction(RT - PCR)method was used to investigate the expression level of OPN and MMP - 9 in the endometrial epithelial cells. Results The expression levels of OPN and MMP - 9 in mifepristone group both in eutopic and ectopic endometrium were lower than those in control group(P ﹤ 0. 05). The mRNA expression levels of OPN and MMP - 9 in endometrium epithelial cells after the intervention of mifepristone at a dose of 0. 1 and 1. 0 mmol/ L were lower than those at 0 mmol/ L( P ﹤ 0. 05);the mRNA expression levels of OPN and MMP - 9 in endometrium epithelial cells after the intervention of mifepristone at a dose of 1. 0 and 10. 0 mmol/ L were higher than those at 0. 1 mmol/ L( P ﹤ 0. 05);the mRNA expression levels of OPN and MMP - 9 in endometrium epithelial cells after the intervention of mifepristone at the dose of 10. 0 mmol/ L were higher than those at 1. 0 mmol/L(P ﹤ 0. 05). Conclusion Mifepristone can obviously lower the expression levels of OPN and MMP - 9 in endometrium of endometriosis,and thus inhibit the invasion,adhesion and implantation metastasis of endometrium.关键词
子宫内膜异位症/米非司酮/骨桥蛋白质/基质金属蛋白酶 9Key words
Endometriosis/Mifepristone/Osteopontin/Matrix metalloproteinase 9分类
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杨眉,蒋春樊,哈春芳..米非司酮对子宫内膜异位症子宫内膜腺上皮细胞骨桥蛋白和基质金属蛋白酶9表达的影响[J].中国全科医学,2016,19(24):2925-2929,5.
