中国全科医学2016，Vol.19Issue(24)：2943-2947,5.DOI:10.3969/j.issn.1007-9572.2016.24.015
大黄素治疗重症急性胰腺炎肺损伤机制研究
Mechanism of Emodin in the Treatment of Lung Injury Induced by Severe Acute Pancreatitis
摘要
Abstract
Objective To investigate the mechanism of emodin( EMO)in the treatment of lung injury induced by severe acute pancreatitis lung injury(SAPLI). Methods From June 2014 to December 2015,90 SPF grade healthy male SD rats were selected and divided into SHAM operation group(SHAM group,n = 30),SAPLI group(n = 30),and EMO group(n= 30)by random number table method. In SAPLI group and EMO group,retrograde injection of 4% sodium taurocholate through the biliopancreatic duct was conducted to prepare SAPLI rat models,and SHAM group was injected with the same volume of 0. 9% sodium chloride solution. One hour after model building,EMO group received intraperitoneal injection of emodin,and SAPLI group and SHAM group were injected with the same volume of 0. 9% sodium chloride solution. 24 hours after intervention, 11 rats died in SAPLI group,and 4 rats died in EMO group. Lung wet to dry weight ratio,histopathology scores,MPO level, the serum levels of TNF-α,IL-6 and IL-10,Treg expression rate and Foxp3 mRNA expression were determined. Results SAPLI group and EMO group were higher than SHAM group in lung wet to dry weight ratio and histopathology score(P ﹤ 0. 05);EMO group was lower than SAPLI group in lung wet to dry weight ratio and histopathology score(P ﹤ 0. 05). SAPLI group and EMO group were higher than SHAM group in MPO level(P ﹤ 0. 05);EMO group was lower than SAPLI group in MPO level(P ﹤0. 05). SAPLI group and EMO group were higher in the levels of TNF-α and IL-6 and lower in the level of IL-10 than SHAM group(P ﹤ 0. 05);EMO group was lower in the levels of TNF-α and IL-6 and higher in the level of IL-10 than SAPLI group(P﹤ 0. 05). SAPLI group and EMO group were higher than SHAM group in the expression rate of Treg and the expression of Foxp3 mRNA(P ﹤ 0. 05);EMO group was higher than SAPLI group in the the expression rate of Treg and the expression of Foxp3 mRNA(P ﹤ 0. 05). Conclusion EMO is effective in the treatment of SAPLI,and the mechanism may be increasing Treg expression rate and its functional activity,lowering the levels of MPO,TNF-α and IL-6,increasing the level of IL-10,and therefore playing a protective role for lung tissue.关键词
胰腺炎/肺损伤/大黄素/T 淋巴细胞,调节性Key words
Pancreatitis/Lung injury/Emodin/T - lymphocytes,regulatory分类
医药卫生引用本文复制引用
杨宝晶,尤胜义,张志远,马涛,赵欣..大黄素治疗重症急性胰腺炎肺损伤机制研究[J].中国全科医学,2016,19(24):2943-2947,5.基金项目
天津市科技计划支撑项目 （）
