中国全科医学2016，Vol.19Issue(24)：2948-2952,5.DOI:10.3969/j.issn.1007-9572.2016.24.016
六君子汤提取物抑制食管癌 Ec9706细胞增殖及其对信号转导和转录活化因子3信号通路的影响
Inhibition of Extract of Liujunzi Decoction on Ec9706 Cells Growth of Esophageal Carcinoma and Its Effects on Signaling Pathway of Signal Transducers and Activations of Transcripition 3
摘要
Abstract
Objective To observe the inhibition of extract of Liujunzi decoction on esophageal carcinoma cells Ec9706 growth and the effects on signaling pathway of signal transducers and activations of transcripition 3(STAT3). Methods From 2013 to 2015,Liujunzi decoction was extracted by 70% ethanol,overnight extraction was made by normal butanol,ethyl acetate and water extraction. MTT method was applied to observe the influence of the extracts on the activity of Ec9706 cells. The optimal extracting parts,and enzyme linked immunosorbent assay( ELISA) was used to observe the effects of ethyl acetate extract in Liujunzi decoction on the related cytokines〔 including interleukin - 6( IL-6),transforming growth factor - β1( TGF-β1), vascular endothelial growth factor(VEGF)〕secreted by Ec9706 cells. Western - blotting method was adopted to test the effects of ethyl acetate extract in Liujunzi decoction on protein expression of esophageal carcinoma cells Ec9706 STAT3 and phosphorylation STAT3(p-STAT3). Results There was significant difference in OD value of Ec9706 cells among the control group,70%ethanol parts,normal butanol parts,and water - extraction parts of Liujunzi decoction( F = 448. 90,P ﹤ 0. 01 ) . The inhibition effects of Ec9706 cell growth in all medication groups were shown as follows:normal butanol parts of Liujunzi decoction﹥ 70% ethanol parts of Liujunzi decoction ﹥ water - extraction part of Liujunzi decoction. There was significant difference in OD value of Ec9706 cells in ethyl acetate parts of Liujunzi decoction( F = 19. 78,P ﹤ 0. 001). When the concentration was 250μg/ ml,the inhibition ratio of 70% ethanol parts,normal butanol parts,and water - extraction parts of Liujunzi decoction on Ec9706 were 7. 31% ,23. 24% ,and - 25. 07% respectively,while the concentration was 200 μg/ ml,the inhibition rate of the ethyl acetate extract of Liujunzi decoction on Ec9706 cells was 77. 03% ,showing a inhibitory effect that was significantly better than the other parts,so the filtered effective part was ethyl acetate fraction of Liujunzi decoction. The inhibition rates IC35 ,IC50 , IC70 of ethyl acetate part of Liujunzi decoction calculated by probabilistic method represented low, medium and high concentrations respectively,and the corresponding concentration were 63. 08,93. 00 and 133. 70 μg/ ml. There was significant difference in the levels of IL-6,TGF-β1,and VEGF among the control group,low concentration and medium concentration of Liujunzi decoction groups(P ﹤ 0. 01);the levels of IL-6,TGF-β1,and VEGF in low concentration and medium concentration of Liujunzi decoction groups were lower than those in control group(P ﹤ 0. 05). The expression levels of STAT3 and p-STAT3 in control group and low concentration and medium concentration of Liujunzi decoction groups were significantly different( P ﹤0. 05);the expression levels of STAT3 and p-STAT3 in the low concentrations and medium concentration of Liujunzi decoction groups were lower than those in the control group(P ﹤ 0. 05). Conclusion Among the extracts of different reagents in Liujunzi decoction,the ethyl acetate extraction has the best inhibitory effect on the proliferation of esophageal carcinoma cells,and the inhibition effect is probably related to the adjusting of STAT3 signaling pathway.关键词
食管肿瘤/六君子汤/提取物/乙酸乙酯/STAT3Key words
Esophageal neoplasms/Liujunzi decoction/Extracts/Ethyl acetate/STAT3分类
医药卫生引用本文复制引用
崔姗姗,高小玲,王慧慧,吴耀松,尹素改,陈玉龙..六君子汤提取物抑制食管癌 Ec9706细胞增殖及其对信号转导和转录活化因子3信号通路的影响[J].中国全科医学,2016,19(24):2948-2952,5.基金项目
国家自然科学基金资助项目（81173177）；河南省教育厅自然科学基金资助项目 （）
