中国药房2016，Vol.27Issue(22)：3034-3036,3.DOI:10.6039/j.issn.1001-0408.2016.22.04
转铁蛋白修饰长春新碱-粉防己碱脂质体中主药含量及包封率的测定
Determination of Content and Entrapment Efficiency of Transferrin Modified Vincristine-Tetrandrine Lipo-somes
摘要
Abstract
OBJECTIVE:To establish the method for the determination of main components content and entrapment efficiency of transferrin modified vincristine-tetrandrine liposomes. METHODS:HPLC method was adopted to determine the content of vin-cristine. The determination was performed on ELITE C18 column with mobile phase consisted of methanol-15%triethylamine (70∶30)at a flow rate of 1.0 ml/min. The determination wavelength was set at 297 nm and column temperature was 30 ℃. The sample size was 20 µl. Free drug was isolated from liposomes by dextran gel column chromatography,and entrapment efficiency was deter-mined. RESULTS:The linear range of vincristine was 160-1 600 μg/ml (r=0.999 8,n=5) with average recovery of 99.20%(RSD=0.26%,n=9). RSD of precision test was 0.070%(n=5). The average content of vincristine in liposomes was 0.790 mg/ml(RSD=0.15%,n=3),and the average entrapment efficiency was 85.94%(RSD=2.08%,n=3). CONCLUSIONS:The method is accurate,reliable,simple and rapid,and can be used for the determination of the content and entrapment efficiency of vincristine in transferrin modified vincristine-tetrandrine liposomes.关键词
长春新碱/粉防己碱/转铁蛋白/脂质体/含量测定/包封率/高效液相色谱法Key words
Vincristine/Tetrandrine/Transferrin/Liposomes/Content determination/Entrapment efficiency/HPLC分类
医药卫生引用本文复制引用
李学涛,唐炜,姜英,程岚..转铁蛋白修饰长春新碱-粉防己碱脂质体中主药含量及包封率的测定[J].中国药房,2016,27(22):3034-3036,3.基金项目
国家自然科学基金资助项目（No.81541081）；辽宁省科学技术计划项目（No.2014020046）；辽宁省教育厅重点实验室基础研究项目 （）
