中国药房2016，Vol.27Issue(22)：3054-3057,4.DOI:10.6039/j.issn.1001-0408.2016.22.10
荧光光谱法测定3种黄酮类化合物与人血清白蛋白相互作用的机制研究
Study on Interaction Mechanism between 3 Kinds of Flavonoids Compounds and Human Serum Albumin by Fluorescence Spectrometry
摘要
Abstract
OBJECTIVE:To study the interaction mechanism between flavonoids and human serum albumin (HSA),and to compare the effects of different B-ring substitutions(hydroxyl,methoxyl group)of flavonoids on macromolecular receptor. METH-ODS:The interaction regularity between three flavonoids with different B-ring substitutions(quercetin,hesperetin,methyl hespere-tin) and HSA was studied with fluorescence spectroscopy,the fluorescence quenching types between 3 flavonoids and HSA were determined and analyzed,and the velated binding constant,binding site and thermodynamic parameters were calculated. RE-SULTS:The quenching constants (Ksv) and binding constants (KA) were decreased with the increase of temperatures. The number of binding site(n)was approximately equal to one,and the thermodynamic parameters ΔH<0,ΔS>0,the binding interaction of these compounds with macromolecules was influenced because of the difference of the B-ring substituents. CONCLUSIONS:The quenching mechanism between three flavonoids and HSA was static quenching;the number of binding site was one;the interaction force of the three compounds with HSA was mainly static electricity,and hydroxyl group in the B-ring was likely the major active group and exerted stronger binding force than methoxyl group to connect with macromolecules.关键词
黄酮/荧光猝灭/人血清白蛋白/机制Key words
Flavonoids/Fluorescence quenching/Human serum albumin/Mechanism分类
数理科学引用本文复制引用
兰蕊,龚小保,黄利挂,陈竹,曾雪,张保顺..荧光光谱法测定3种黄酮类化合物与人血清白蛋白相互作用的机制研究[J].中国药房,2016,27(22):3054-3057,4.基金项目
重庆市基础与前沿研究计划项目（No.cstc2013jcy-jA10070，cstc2014jcyjA10125）；西南大学基本科研业务费专项资金项目（No.XDJK2013B040）；重庆市教委科学技术研究项目（No. KJ132501）；重庆市卫生局医学科研项目 （）
