中国药房2016，Vol.27Issue(22)：3066-3068,3.DOI:10.6039/j.issn.1001-0408.2016.22.13
HPGPC法测定羧基麦芽糖铁重均分子质量和分子质量分布
Determination of Weight Average Molecular Weight and Molecular Weight Distribution of Ferric Carboxy-maltose by HPGPC
摘要
Abstract
OBJECTIVE:To establish a method for the determination of weight average molecular weight(Mw)and molecular weight distribution (D) of ferric carboxymaltose. METHODS:HPGPC method was adopted to detect the Mw and D of 3 batches of Ferric carboxymaltose injection (imported) and its raw material (self-made). The determination was performed on TSK G4000 PWXL column with 0.1% sodium azide solution with the flow rate of 0.5 ml/min. The detector was refractive index detector;the column temperature was set at 35 ℃,and sample size was 20 µl. The results were calculated with GPC software. RESULTS:RSDs of precision,stability and reproducibility tests were all lower than 3.0%(n=6);Mw and D of 3 imported samples were 157 667 and 1.30;those of self-made samples were 162 000 and 1.42. CONCLUSIONS:The method has high precision,good stability,repeat-ability and durability. It can be used for the determination of Mw and D of ferric carboxymaltose.关键词
高效凝胶渗透色谱法/羧基麦芽糖铁/重均分子质量/分子质量分布Key words
HPGPC method/Ferric carboxymaltose/Weight average molecular weight/Molecular weight distribution分类
医药卫生引用本文复制引用
李蔷薇,李强,郑筠,周亮..HPGPC法测定羧基麦芽糖铁重均分子质量和分子质量分布[J].中国药房,2016,27(22):3066-3068,3.基金项目
药食同源植物资源开发四川省高校重点实验室开放基金项目 （）
