中国药理学通报2016，Vol.32Issue(8)：1063-1067,5.DOI:10.3969/j.issn.1001-1978.2016.08.007
山茱萸环烯醚萜苷类成分对 AGEs诱导 HUVEC 损伤的保护作用
Protective effect of loganin and morroniside on HUVEC injury induced by advanced glycation end products
摘要
Abstract
Aim To observe the protective mechanism of loganinand morroniside ( active components in Cornus officinalis) on HUVEC injury induced by advanced glycation end products ( AGEs ) .Methods HUVECs were cultured in vitro and divided into control group , model group ( AGEs group ) , loganin group , morroni-side group and aminoguanidine group ( set as positive control).After being incubated with loganin and mor-roniside( final concentrations were 100,10,1 μmol・L-1 ) for 1 h, HUVECs were stimulated by AGEs of 200 mg・ L-1 for 24 h.Then, the cell viability was measured by using MTT method .The supernatant was extracted and the levels of NO ,ET-1,MCP-1,VCAM-1 were measured by the corresponding kits .Receptors of advanced glycation end products ( RAGE ) and NF-κB in HUVEC were detected by Western blot .Results Loganin and morroniside could inhibit HUVEC injury induced by AGEs .In model group ,the contents of ET-1,MCP-1,VCAM-1 increased(P<0.01),the content of NO decreased ( P <0.01 ) and the expression of RAGE and NF-κB increased(P<0.01); however,lo-ganin and morronside could reduce the ET-1,MCP-1, VCAM-1contents,increase the NO content and down-regulate the expression of RAGE and NF-κB to differ-ent extents .Conclusion Loganin and morroniside could ameliorate HUVEC injury , and its mechanism may be related to inhibit inflammation , the improve-ment of endothelial cell function , and the decrease of the expression of RAGE .关键词
山茱萸/马钱苷/莫诺苷/糖尿病/AGEs/HUVECKey words
Cornus officinalis Sieb.et Zucc./loga-nin/morroniside/diabetes mellitus/AGEs/HUVEC分类
医药卫生引用本文复制引用
沈红胜,许惠琴,陆春红,戴国英,徐康,吕兴,陈玉萍,吴云皓..山茱萸环烯醚萜苷类成分对 AGEs诱导 HUVEC 损伤的保护作用[J].中国药理学通报,2016,32(8):1063-1067,5.基金项目
国家自然科学基金资助项目（No 81374029，81073111）；江苏高校优势学科建设工程资助项目 （）
