中国药理学通报2016，Vol.32Issue(8)：1068-1074,7.DOI:10.3969/j.issn.1001-1978.2016.08.008
桃叶珊瑚苷通过 ERβ途径抑制 TNF－α诱导的心脏祖细胞凋亡
Aucubin inhibited apoptosis of mouse cardiac progenitor cells induced by TNF-αthrough ERβpathway
摘要
Abstract
Aim Aucubin(AU) is one of the effective ingredients of eucommia , plantain , rehmannia and oth-er herbs.Modern pharmacological studies have shown that AU has protective effects on immunity , cardiovas-cular and nervous system , but its mechanism is un-clear .Previous studies have confirmed that AU can ac-tivate estrogen receptor , suggesting that AU may play a role in cardiovascular protection through estrogen signa-ling pathway .In recent years , more and more evidence showed that cardiac progenitor cells ( CPCs ) play an important role in the repair of myocardial ischemic in-jury.Methods To further elucidate the mechanism , our study used mouse CPCs as cell model and elucida-ted the effect of AU on CPCs apoptosis induced by tumor necrosis factor alpha ( TNF-α) and its mecha-nism by IncuCyte live cell imaging , TUNEL staining , Western blot and quantitative PCR . Results AU could reduce TNF-α-induced apoptosis of CPCs , de-crease the expression of caspase-3 and up-regulate Bcl-2/Bax levels.Estrogen receptor beta ( ERβ) antagonist could block the anti-apoptotic effect of AU , and AU treatment was able to increase the expression of ERβ. Conclusion AU could inhibit the apoptosis of CPCs induced by TNF-α, and its mechanism is the activation of ERβpathway .关键词
桃叶珊瑚苷/心脏祖细胞/雌激素受体β/肿瘤坏死因子-α/细胞凋亡/组织损伤修复Key words
aucubin/cardiac progenitor cells/estro-gen receptor β/tumor necrosis factor-α/cell apopto-sis/tissue injury repair分类
医药卫生引用本文复制引用
李春晓,李慧影,王虹,陈璐,邱丽珍,耿潇,尤星宇..桃叶珊瑚苷通过 ERβ途径抑制 TNF－α诱导的心脏祖细胞凋亡[J].中国药理学通报,2016,32(8):1068-1074,7.基金项目
新世纪优秀人才支持计划（ No NCET－13－0935）；国家自然科学基金资助项目（ No 81173592）；国家国际科技合作专项（No 2015DFA30430）；长江学者和创新团队发展计划资助项目 （）
