中国药理学通报2016，Vol.32Issue(8)：1086-1090,5.DOI:10.3969/j.issn.1001-1978.2016.08.011
组织蛋白酶B在SAHA诱导乳腺癌MCF－7细胞凋亡过程中的调控作用
The regulation of Cathepsin B on cell apoptosis induced by SAHA in breast cancer cell line MCF-7
摘要
Abstract
Aim To clarify the regulation role of ca-thepsin B ( Cat B ) in cell proliferation and apoptosis induced by SAHA in ER-positive breast cancer cell line MCF-7.Methods MTT was used to screen the optimal concentration and treatment time of SAHA . The expression levels of related proteins were deter-mined by ELISA , and the morphological changes were observed through time-lapse live cell imaging acquisi-tion.Cell viability and apoptosis assay in MCF-7 cells were assessed by Muse Cell Analyzer with SAHA and /or Cystatin C treatment .Results MTT assay showed that the anti-tumor efficacy of SAHA was significant . The optimal concentration and treatment time were 10μmol・ L-1 and 24 h respectively . ELISA assay showed that SAHA could induce expression of Cat B in MCF-7 cells.Real-time live-cell imaging experiments demonstrated that the combination treatment of Cystatin C and SAHA significantly resumed the inhibitory effect caused by SAHA alone .Cytology test showed that SA-HA alone obviously depressed the cell viability and in-duced apoptosis . However , the effect was reversed with the combination of Cystatin C .Conclusion Cat B plays an important role in apoptosis induced by SAHA in ER +breast cancer cells MCF-7.关键词
乳腺癌/雌激素受体阳性细胞/MCF-7/组织蛋白酶B/SAHA/细胞凋亡Key words
breast cancer/estrogen receptor positive cell line/MCF-7/Cat B/SAHA/cell apoptosis分类
医药卫生引用本文复制引用
李静,周伟强..组织蛋白酶B在SAHA诱导乳腺癌MCF－7细胞凋亡过程中的调控作用[J].中国药理学通报,2016,32(8):1086-1090,5.基金项目
国家自然科学基金资助项目（No 81172509）；辽宁省自然科学基金资助项目 （）
