中国药理学通报2016，Vol.32Issue(8)：1110-1114,5.DOI:10.3969/j.issn.1001-1978.2016.08.016
白藜芦醇抑制人结肠癌细胞增殖与 p38 MAPK 的关系研究
Anti-proliferation effect of resveratrol and p38 MAPK in human colon cancer cells
摘要
Abstract
Aim To investigate the relationship be-tween the anti-proliferation effect of resveratrol ( Res ) and p38 MAPK in colon cancer cells .Methods Crys-tal violet staining , Western blot and flow cytometry were employed to analyze the effect of Res on the pro-liferation in LoVo cells.Western blot assay was used to detect the effect of Res on the apoptosis of LoVo cells and the phosphorylation of p 38 MAPK.Crystal violet staining and Western blot assay were used to analyze whether p38 MAPK was involved in the Res-induced proliferation inhibition and apoptosis in LoVo cells .Re-sults Res inhibited the proliferation , arrested cell cy-cle at S phase , and increased the protein level of PC-NA in LoVo cells apparently .Res increased the level of Bad in LoVo cells, but decreased the level of Bcl-2. Although Res exerted no substantial effects on total lev-el of p38 MAPK, it markedly increased the phospho-rylation level of p38 MAPK in LoVo cells.p38 MAPK inhibitor promoted the proliferation , and decreased the anti-proliferation effect of Res on LoVo cells .Moreo-ver , the effects of Res on the level of Bcl-2 and Bad were both reduced by the p 38 MAPK inhibitor .Con-clusions Res can inhibit the proliferation of LoVo cells, which may be partly mediated by promoting the phosphorylation of p38 MAPK.关键词
白藜芦醇/结肠癌/LoVo细胞/增殖抑制/凋亡/p38 MPAKKey words
resveratrol/colon cancer/LoVo cells/an-ti-proliferation/apoptosis/p38 MAPK分类
医药卫生引用本文复制引用
陈前昭,曾于桦,邵英,李洋,任文艳,周林云,周益,刘荣兴,何百成..白藜芦醇抑制人结肠癌细胞增殖与 p38 MAPK 的关系研究[J].中国药理学通报,2016,32(8):1110-1114,5.基金项目
国家自然科学基金资助项目 （）
