中国药理学通报2016，Vol.32Issue(8)：1170-1174,5.DOI:10.3969/j.issn.1001-1978.2016.08.027
泛素连接酶Cbl－b调控p38MAPK在胰岛素与硒协同抑制糖尿病心肌病大鼠心肌细胞凋亡中的作用
Role of ubiquitin ligase Cbl-b-regulated p38MAPK in insulin and selenium synergistic anti-myocardial apoptosis in diabetic cardiomyopathy
摘要
Abstract
Aim To explore the mechanism of insulin in combination with selenium preventing myocardial apoptosis in diabetic cardiomyopathy rats .Methods SD rats ( n =50 ) were randomly divided into five groups: control , diabetic cardiomyopathy ( DCM ) , DCM with insulin treatment , DCM with selenium treat-ment, and DCM with insulin and selenium combination treatment .The cell apoptosis was observed by TUNEL . The levels of Bcl-2, caspase-3, PARP, Cbl-b and p38 MAPK were examined by Western blot .The inter-actions of Cbl-b-p38 MAPK and Ku70-Bax were detec-ted by immunoprecipitation .Results Insulin in com-bination with selenium synergistically inhibited apopto-sis, up-regulated Cbl-b, down-regulated p38MAPK ex-pressions and increased the interactions of Cbl-b-p38MAPK and Ku70-Bax.Conclusion Insulin and selenium synergistically inhibit myocardial apoptosis by regulating Cbl-b-inhibited p38 MAPK and preventing Bax translocation .关键词
胰岛素/硒/糖尿病心肌病/凋亡/Cbl-b/p38 丝裂原活化蛋白激酶/Ku70Key words
insulin/selenium/diabetic cardiomyopa-thy/apoptosis/Cbl-b/p38 MAPK/Ku70分类
医药卫生引用本文复制引用
徐天娇,刘勇,李萍,胥晓丽,曾菊绒..泛素连接酶Cbl－b调控p38MAPK在胰岛素与硒协同抑制糖尿病心肌病大鼠心肌细胞凋亡中的作用[J].中国药理学通报,2016,32(8):1170-1174,5.基金项目
陕西省教育厅自然科学项目（ No 15 JK1633）；西安医学院重点学科建设经费资助 （）
