浙江医学2016，Vol.38Issue(15)：1225-1229,5.
蛛网膜下腔注射 SRT1720对慢性坐骨神经结扎大鼠脊髓SIRT1表达和NF-κB去乙酰化调节的影响
Effects of intrathecal SRT1720 injection on expressions of SIRT1 and deacetylation of NF-κB in spinal cord in rats with chronic constriction injury
摘要
Abstract
Objective To investigate the effect of intrathecal SRT1720 injection on expression and activity of SIRT1, and deacetylation of NF- κBp65 in rats with chronic constriction injury (CCI). Methods One hundred and twenty male SD rats were randomly divided into five groups with 24 animals in each group:Sham group (S group), CCI group, dimethylsulfoxide (DMSO) group, CCI+LD group and CCI+HD group. Lumbosacral intrathecal (IT) catheter was placed 8d before CCI operation in DMSO, CCI+LD and CCI+HD groups. CCI was induced in al animals except those in S group. 10μl DMSO was intrathecal y injected q.d via the catheter in DMSO group; 10μg and 20μg SRT1720 dissolved in 10μl DMSO was given to rats in CCI+LD and CCI+HD groups. Six rats were sacrificed randomly before and at 1, 3, 7 d after CCI or sham surgery in each group. The L4~5 spinal cord segments were removed for detection of deacetylase activity of SIRT1 by fluorometric kit. The proteins levels of (SIRT1) and NF- κB/Ac- NF- κB p65 were determined by Western blot analysis. Results The deacetylase activity and protein levels of SIRT1 were significantly decreased after operation in CCI and DMSO group at postoperative d1, 3 and 7, and the proteins level of NF- κB/Ac- NF- κB p65 were significantly increased(P<0.01), compared with S group(P<0.01). Compared with CCI group, the deacetylase activity and protein level of SIRT1 increased, and NF- κB/Ac- NF- κB p65 protein level decreased both in CCI+LD group and CCI+HD group at postoperative d3 and d7 (P<0.01). Furthermore, the activity and protein levels of SIRT1 were higher in CCI+HD group and the proteins levels of Ac- NF- κB p65 were lower (P<0.01) than those in CCI+LD group at postoperative d7 (P<0.01). Conclusion SRT1720 intrathecal injection can improve the down- regulation of spinal SIRT1 deacetylase activity and protein levels in CCI rats, furthermore, it also can inhibit over- expression and acetylation of NF- κB p65.关键词
SRT1720/慢性坐骨神经结扎/沉默信息调节因子2相关酶1/NF- κB去乙酰化Key words
SRT1720/Chronic constriction injury/Silentmating type information regulation 2 homolog 1/NF- κB Deacetylation引用本文复制引用
吕晨,郭小文,郑晖,叶玲,马千,张娟..蛛网膜下腔注射 SRT1720对慢性坐骨神经结扎大鼠脊髓SIRT1表达和NF-κB去乙酰化调节的影响[J].浙江医学,2016,38(15):1225-1229,5.基金项目
国家自然科学基金青年科学基金项目（81202823）；浙江省医药卫生科技计划项目 （）
