浙江医学2016，Vol.38Issue(15)：1234-1237,4.
水杨酸抑制胃癌细胞体外增殖及侵袭转移能力研究
Effect of salicylic acid on adhesion, migration and invasion of human gastric cancer cells
葛曙雄 1刘雅辉 2张立 1李科事 1胡岳 2王涌2
作者信息
- 1. 315211 宁波大学医学院
- 2. 宁波市第一医院重点实验室，普外科
- 折叠
摘要
Abstract
Objective To investigate the effects of salicylic acid on adhesion, migration and invasion abilities of gastric cancer cel line and their mechanisms. Methods Human gastric cancer cel s were incubated with different concentrations of salicylic acid. The abilities of adhesion, migration and invasion of gastric cancer cel s were evaluated with cel adhesion assay, Transwel migration assay and Transwel invasion assay. The mRNA and protein expression levels of cell adhesion moleculars were tested by Real- time RT- PCR and Western blot, respectively. Results Salicylic acid significantly inhibited the proliferation, adhesion, migration and invasion of gastric cancer cel s in vitro. The mRNA expression levels of Lamin, LaminC1, LaminB1, FN1, CD44, ITGA9 and CTNNA1 were decreased;however, expression of CDH1 was increased after salicylic acid treatment. While the protein expression levels of LaminB1, FN1, CD44 and CTNNA1 were also decreased(P<0.05). Conclusion Salicylic acid inhibits the proliferation, adhesion, migration and invasion of gastric cancer cel , which is associated with the expression of cell adhe-sion- related molecules and the resistance to epithelial- mesenchymal transition.关键词
水杨酸/胃癌细胞株/侵袭转移/细胞黏附分子Key words
Salicylic acid/Gastric cancer cel line/Migration and invasion/Cel adhesion molecules引用本文复制引用
葛曙雄,刘雅辉,张立,李科事,胡岳,王涌..水杨酸抑制胃癌细胞体外增殖及侵袭转移能力研究[J].浙江医学,2016,38(15):1234-1237,4.
