浙江医学2016，Vol.38Issue(15)：1241-1245,5.
TGF-β1诱导胃癌细胞上皮间质转化与CD133表达研究
TGF-β1 induces epithelial-mesenchymal transition in gastric cancer cells and its mechanism
钟志凤 1杜金林 1王建平 1金晰函 1戴剑1
作者信息
- 1. 321000 金华市中心医院结直肠肛门外科
- 折叠
摘要
Abstract
Objective To investigate the effect of TGF- β1 on the epithelial- mesenchymal transition (EMT) in gastric cancer cel s and its mechanism. Methods Human gastric cancer MKN- 45 cel s were treated with TGF- β 1. The micromorphological changes were observed and the invasive potency of MKN- 45 cel s were examined with Transwel method;the mRNA and protein expressions of Snail, E- cadherin, N- cadherin, p- Akt and CD133 were detected by RT- PCR and Western blot, respectively. The MKN- 45 cel s were pretreated with PI3K special inhibitor LY294002, then treated with TGF- β1, and the expressions of p- Akt and CD133 were detected. CD133+and CD133- cel s were sorted by MACS and the expression of EMT- related proteins were measured and compared between two subsets of MKN- 45 cel s. Results The micromorphology of culture cells was changed to mesenchymal profiles 72h after TGF- β1 treatment;and the mRNA and protein expression levels of Snail and N- cadherin were higher than those in control group (P<0.05), the mRNA and protein expression levels of E- cadherin were significantly lower than those in control group (P<0.05). The invasion ability of TGF- β1 treated group was higher than that of control group (P<0.05). The relative protein expression levels of p- Akt and CD133 in TGF- β1 treated group were higher than those in control group(P<0.05). In LY294002 pretreated cel s, the p- Akt and CD133were down- regulated(P<0.05). The relative mRNA and protein expression levels of Snail, N- cadherin in CD133+group were higher than those in CD133-group (P<0.05), the relative mRNA and protein expression levels of E- cadherin in CD133+ group were lower than those in CD133- group(P<0.05). Conclusion TGF- β1 can induce EMT in MKN- 45 cells, enhance the invasion ability of MKN- 45 cel s and up- regulate the expression of CD133 via PI3K/Akt pathway.关键词
TGF- β1/胃/新生物/癌/MKN- 45/上皮间质转化/CD133/PI3K/AktKey words
TGF- β1/Stomach/Neoplasma/cancer/MKN- 45/EMT/CD133/PI3K/Akt引用本文复制引用
钟志凤,杜金林,王建平,金晰函,戴剑..TGF-β1诱导胃癌细胞上皮间质转化与CD133表达研究[J].浙江医学,2016,38(15):1241-1245,5.
