浙江医学2016，Vol.38Issue(15)：1246-1249,4.
右美托咪啶抑制NF-κB活化减轻肢体缺血再灌注损伤的研究
Dexmedetomidine reduces ischemia-reperfusion injury in skeletal muscle via suppressing activation of NF-κB
摘要
Abstract
Objective To investigate the effect of dexmedetomidine (DEX) on skeletal muscle injury induced by ischemia- reperfusion and related mechanisms. Methods Twenty- one SD rats were randomly divided into sham- operated group (sham group), ischemia- reperfusion (I/R) group and I/R+ DEX group. In DEX group, DEX (25μg/kg) was injected after anesthesia;while in the other groups, the same volume of saline was also injected. After 30 minutes, the hind limb ischemia was induced by clamping the common femoral artery and ligating collateral circulation. After 3h of ischemia, the clamp and tourniquet were removed and the rats underwent reperfusion for 2h. The plasma concentrations of IL- 1, IL- 6 and TNF- α were measured with enzyme- linked immunosorbent assay. The gastrocnemius muscle was harvested, the expression of NF- κB protein was detected by Western blot; the morphological changes were observed with HE staining; the wet/dry ratio was also measured. Results The intact muscle fiber and few inflammatory cel s infiltration in interstitial space were observed with light microscopy in sham group. Disordered arrangement and edema of muscle fibers and interstitial inflammatory cells infiltration were showed in I/R group. In DEX group, the disordered arrangement of muscle fiber and infiltration of inflammatory cel s in the stroma were attenuated as compared to I/R group. The wet/dry ratio, plasma levels of TNF- αand NF- κB were higher in I/R group than those in sham group (P<0.05). The wet/dry, plasma IL- 1, IL- 6, TNF- αlevels and NF- κB expression were significantly lower in DEX group than those in I/R group (P<0.05). Conclusion Dexmedetomidine can reduce the ischemia- reperfusion injury of skeletal muscle, which is associated with the inhibition of NF- κB activation.关键词
缺血/再灌注损伤/NF-κB/右美托咪啶Key words
Reperfusion injury/NF- κB/Dexmedetomidine引用本文复制引用
郑艳雅,温新意,袁培根,林丽娜,倪纯珏,赵喜越..右美托咪啶抑制NF-κB活化减轻肢体缺血再灌注损伤的研究[J].浙江医学,2016,38(15):1246-1249,4.基金项目
温州市科技计划项目 （）
