作物学报2016，Vol.42Issue(8)：1112-1121,10.DOI:10.3724/SP.J.1006.2016.01112
马铃薯HD-Zip I家族ATHB12基因的克隆及功能鉴定
Cloning and Functional Identification of theATHB12Gene of HD-Zip IFamily in Potato (Solanum tuberosum L.)
摘要
Abstract
HD-Zip I is a class of plant-specific transcription factors, which has an important role in response to adversity stress in plant. AATHB12 gene of HD-Zip I transcription factors was cloned from potato cultivar Gannongshu 2, which contains a 759 bp open reading frame (ORF) encoding a protein of 252 amino acid residues.ATHB12 gene is located on potato chromosome 1, and its promoter region sequence contains cis-acting elements including ABRE, LTRECOREATCOR15, WBOXATNPR1 responsive to abiotic stresses (ABA, temperature, dehydration, and salt stress).ATHB12 gene expressed in root, stem and leaf of potato, with the highest expression in the root. qRT-PCR analysis confirmed that the gene was induced by PEG, NaCl, and ABA, but repressed by cold treatment. The overexpressed-vector ofATHB12 gene driven by the constitutive promoter CaMV 35S was constructed, and the transgenic plants were obtained usingAgrobacterium-mediated transformation system. The malondialdehyde (MDA) content in the transgenic plant leaves was significantly lower (P< 0.05), whereas the proline content was significantly higher (P<0.05) than those of non-transgenic control under drought stress. The fresh and dry weight of the transgenic plant root was higher than that of non-transgenic plants. These results showed thatATHB12 gene may be involved in response to stress.关键词
马铃薯/HD-Zip/ATHB12基因/根/丙二醛/脯氨酸Key words
Potato/HD-Zip/ATHB12 gene/Root/Malondialdehyde/Proline引用本文复制引用
武亮亮,姚磊,马瑞,朱熙,杨江伟,张宁,司怀军..马铃薯HD-Zip I家族ATHB12基因的克隆及功能鉴定[J].作物学报,2016,42(8):1112-1121,10.基金项目
本研究由国家自然科学基金项目(31460370),高等学校博士学科点专项科研基金项目(20126202110007),国家国际科技合作专项(0102014DFG31570)和甘肃省干旱生境作物学省部共建国家重点实验室培育基地开放基金项目(GSCS-2012-02)资助。 This study was supported by the National Natural Science Foundation of China (31460370), Specialized Research Fund for the Doctoral Program of Higher Education of China (20126202110007), International Science & Technology Cooperation Program of China (0102014DFG31570), and Gansu Key Laboratory of Aridland Crop Science of Gansu Agricultural University (GSCS-2012-02) （31460370）
