作物学报2016，Vol.42Issue(8)：1122-1133,12.DOI:10.3724/SP.J.1006.2016.01122
玉米抗禾谷镰刀菌的转录组分析
Transcriptional Analysis of Maize Resistance against Fusarium graminearum
摘要
Abstract
Gibberella stalk rot, caused byFusarium graminearum (teleomorph,Gibberella zeae), is one of the most devastating soil-borne diseases in maize. It seriously decreases maize yield and quality. Molecular mapping led to the identification of two QTLs,qRfg1 and qRfg2, on chromosomes 10 and 1 respectively, conferring resistance toGibberella stalk rot. In order to charac-terize the defense mechanism of maize againstF. graminearum, NIL-R with resistant alleles at both QTLs and NIL-S with the susceptible alleles at both QTLs were generated and used in transcriptome analysis. After inoculation of young seedling roots of both NILs with theF. graminearumspores, the inoculated roots were sampled at 0, 6, and 18 hours after inoculation (hai) for transcriptome analysis using RNAseq. The basal difference was achieved by the comparison between control samples. In total, 2958 genes were differentially expressed between control samples of NIL-R and NIL-S, among which 1170 genes were more abundant in NIL-R. GO analysis revealed that genes involved in biological processes related to JA/ET and SA biosynthesis, JA/ET mediated signaling pathway and SA mediated signaling pathway were significantly enriched. Phenylpropanoid biosynthesis proc-ess was enriched in the genes more abundant in NIL-R and genes encoding enzymes involved in phenylpropanoid biosynthesis like PAL, 4CL2, CAD, and HCTwere more abundant in NIL-R. There were 431 genes differentially expressed between NIL-R and NIL-S at 6 hai, among which 83 genes were more abundant in NIL-R. Genes encoding pathogenesis-related (PR) proteins like lipid-transfer protein and germin-like proteinwere more abundant in NIL-R. Among the 1292 genes differentially expressed be-tween NIL-R and NIL-S. At 18 hai, 291 genes were more abundant in NIL-R. Genes involved in ET biosynthesis likeACO and JA biosynthesis likeLOXwere more abundant in NIL-R. Genes involved in DON detoxification likePDR1 andMDR2 were more abundant in NIL-R. After inoculation withF. graminearum, 428 genes were exclusively up-regulated in NIL-R at 6 hai compared with control. Genes involved in ET biosynthesis and ET-mediated signaling pathway like ACO,ERF,EBF1, andEIL1 and patho-genesis-related genes likePR1,OSM34, andgermin-like proteinwere exclusively up-regulated in NIL-R. At 18 hai, 359 genes were exclusively up-regulated in NIL-R compared with control. Pathogenesis-related genes likePR1,PR4, and genes encoding the transporters of DON out of cytoplasm likeABC transport family protein, heavy metal transport protein and MATE efflux fam-ily protein were exclusively up-regulated in NIL-R. All these results indicate that NIL-R can increase the resistance of maize toF. graminearum by the constitutive resistance characterized by the higher expression of genes related to defense responses. Genes involved in defense responses exclusively up-regulated in NIL-R and higher expression level of disease resistance genes in NIL-R at 6 and 18 hai may restrict the pathogen invasion after infection. The phenylpropanoid biosynthesis pathway and DON-detoxification proteins identified in this study are important for the resistance againstF. graminearuminfection.关键词
玉米/茎腐病/转录组/抗性/JA/ET/苯丙烷Key words
Maize/Stalk rot/Transcriptome/Resistance/JA/ET/Phenylpropanoid引用本文复制引用
刘永杰,马传禹,马雪娜,徐明良..玉米抗禾谷镰刀菌的转录组分析[J].作物学报,2016,42(8):1122-1133,12.基金项目
本研究由引进国际先进农业科学技术计划(948计划)项目(2003-Q04)资助。 This study was supported by the Program of Introducing International Super Agricultural Science and Technology (948 Program)(2011-G15) （948计划）
