作物学报2016，Vol.42Issue(8)：1134-1142,9.DOI:10.3724/SP.J.1006.2016.01134
小麦矮秆种质SN224的鉴定及农艺性状QTL分析
Identification of Dwarfing Wheat Germplasm SN224 and Analysis of QTLs for Its Agronomic Traits
摘要
Abstract
SN224 is a dwarfing wheat line derived from the cross between hexaploid triticale (AABBRR, 2n= 6x) and common wheat (AABBDD, 2n= 6x). We evaluated its cytologic characteristic and main agronomic traits, and analyzed the genetic basis of dwarfing trait in order to use the germplasm in wheat breeding program.This white-grain wheat had compact plant type, spin-dle-shaped panicle and moderate resistance to powdery mildew (Blumeria graminis f. sp.tritici,Bgt) and stripe rust (Puccinia striiformisf. sp.tritici,Pst). There were 42 chromosomes in root tip cells, showing 21 bivalents in pollen mother cells. FISH con-firmed that SN224 was a 1BL·1RS translocation line. The plant height was between two parents of F1from a cross between SN224 and Huixianhong and distributed continuously and normally in F2 population. The detection of specific molecular marker for genesRht-B1b,Rht-D1b, andRht8 indicated that this line had none of the three dwarfing genes. In the meantime, the introduc-tion of 1RS had no obvious effect on plant height. The F2 population was used to construct a genetic linkage map containing 134 SSR markers which covered a total length of 1332.1 cM. Two major dwarfing QTLs on chromosomes 1B and 4B were detected by additive-inclusive composite interval mapping (ICIM-ADD).QPh1B andQPh4B, located in theXwmc719–Xgwm18 and Xgwm368–Xmag4284intervals, explained 20.0% and 10.2% of phenotypic variation, respectively. Seven QTLs controlling ear length, panicle number per plant, and kernel number per spike were detected. The QTL inKSUM062–Xmag4284interval contrib-uted to decrease plant height, increase ear length and panicle number per plant.关键词
普通小麦-黑麦易位系/农艺性状/细胞学鉴定/QTL定位/矮秆基因Key words
Common wheat-rye translocation line/Agronomic traits/Cytological identification/QTL mapping/Dwarfing gene引用本文复制引用
王鑫,马莹雪,杨阳,王丹峰,殷慧娟,王洪刚..小麦矮秆种质SN224的鉴定及农艺性状QTL分析[J].作物学报,2016,42(8):1134-1142,9.基金项目
本研究由“十二五”国家科技支撑计划项目(2013BAD01B02-8)资助。 This study was supported by the National Key Technology R&D Program of China (2013BAD01B02-8) （2013BAD01B02-8）
