作物学报2016，Vol.42Issue(8)：1253-1258,6.DOI:10.3724/SP.J.1006.2016.01253
小麦wzy2-1基因的克隆及功能分析
Cloning and Functional Analysis ofwzy2-1Gene in Wheat
摘要
Abstract
Dehydrins (DHNs) are identified as the group II of LEA proteins and involved in plant abiotic stress tolerance. In this study, we isolated a novel Kn-type dehydrin gene from wheat cultivar Zhengyin 1, which was designatedwzy2-1. The full length of wzy2-1 is 1740 bp, encoding 579 amino acids and containing nine conserved K-fragments. Sequence alignment indicated that wzy2-1had high homology toDhn5gene inHordeum vulgare. The WZY2-1 protein was predicted to be a highly-hydrophilic and disordered protein. The WZY2-1 protein was successfully expressed inE. coli strain BL21 (DE3). We found that WZY2-1 protein improved the tolerance to low or high temperature, salt and osmotic stresses inE. coli. The qRT-PCR assay indicated that the ex-pression of wzy2-1gene was induced by low temperature, PEG, and salt stresses rather than ABA. Thus, we conclude thatwzy2-1 is an ABA-independent gene.关键词
小麦/脱水素/荧光实时定量PCR/原核表达Key words
Wheat/Dehydrins/Real-time PCR/Procaryotic expression引用本文复制引用
强治全,梁雅珺,于正阳,杜娅,张帅,朱维宁,张林生..小麦wzy2-1基因的克隆及功能分析[J].作物学报,2016,42(8):1253-1258,6.基金项目
本研究由高等学校博士学科点专项科研基金项目(20120204110033)和旱区作物逆境生物学国家重点实验室基金项目(CSBAA2015007)资助。 This study was supported by the Projects for Doctoral Research Funding in Higher Education Institutions Function and Structure of Dehydrin Protein in Different Water Content (20120204110033) and the Foundation of State Key Laboratory of Crop Stress Biology for Arid Areas of Northwest A&F University (CSBAA2015007) （20120204110033）
